PXD023001

PXD023001 is an original dataset announced via ProteomeXchange.

Title	Assessing the biomolecular landscape and dynamics of systemically circulating lipid-carrying particles
Description	Separating lipid-carrying particles from body fluids is challenging and complicates studies of extracellular vesicles (EV) and lipoprotein particles (LPP) as disease biomarkers. In this study, we assess the biomolecular composition and dynamics of systemically circulating matched EV and LPP by analyzing serial blood plasma samples from breast cancer, ovarian cancer and HIV patients (n=36). Particles were separated through combined size-exclusion chromatography (SEC) and equilibrium density gradient centrifugation. After the analytical validity of our method is demonstrated, we reveal that EV carry a unique, dynamic and condition-dependent protein composition and RNA profile, which is not directly measurable in LPP extracts and total blood plasma, respectively. Our study extends the knowledge on the compositional nature of the EV protein corona and provides a catalog of proteins associated with systemically circulating EV and LPP. We anticipate that this research will steer the further development of EV-based biomarkers to detect and monitor disease.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_15:22:12.129.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Delphi Van Haver
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-08 12:08:14	ID requested
⏵ 1	2021-09-09 15:22:12	announced

Vergauwen G, Tulkens J, Pinheiro C, Avila Cobos F, Dedeyne S, De Scheerder MA, Vandekerckhove L, Impens F, Miinalainen I, Braems G, Gevaert K, Mestdagh P, Vandesompele J, Denys H, De Wever O, Hendrix A, Robust sequential biophysical fractionation of blood plasma to study variations in the biomolecular landscape of systemically circulating extracellular vesicles across clinical conditions. J Extracell Vesicles, 10(10):e12122(2021) [pubmed]

submitter keyword: Extracellular Vesicles, Cancer, HIV

An Hendrix
contact affiliation	Laboratory of Experimental Cancer Research, Department of Radiation Oncology and Experimental Cancer Research, Ghent University, Ghent, Belgium. Cancer Research Institute Ghent, Ghent University, Ghent, Belgium.
contact email	An.Hendrix@UGent.be
lab head
Delphi Van Haver
contact affiliation	VIB Proteomics Core
contact email	delphi.vanhaver@vib-ugent.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD023001

PRIDE project URI

• PRIDE
  1. PXD023001
     1. Label: PRIDE project
     2. Name: Assessing the biomolecular landscape and dynamics of systemically circulating lipid-carrying particles