PXD022999

PXD022999 is an original dataset announced via ProteomeXchange.

Title	Proteomics analysis of human intestinal organoids during hypoxia and reoxygenation as a model to study ischemia-reperfusion injury
Description	Intestinal ischemia-reperfusion (IR) injury is associated with high mortality rates, which have not improved in the past decades despite advanced insight in its pathophysiology using in vivo animal and human models. The inability to translate previous findings to effective therapies emphasizes the need for a physiologically relevant in vitro model to thoroughly investigate mechanisms of IR-induced epithelial injury and test potential therapies. In this study, we demonstrate the use of human small intestinal organoids to model IR injury by exposing organoids to hypoxia and reoxygenation (HR). A mass-spectrometry-based proteomics approach was applied to characterize organoid differentiation and decipher protein dynamics and molecular mechanisms of IR injury in crypt-like and villus-like human intestinal organoids.
HostingRepository	PRIDE
AnnounceDate	2021-03-04
AnnouncementXML	Submission_2021-03-04_12:48:00.613.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anna M Kip
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-08 09:25:19	ID requested
⏵ 1	2021-03-04 12:48:00	announced

Dataset with its publication pending

submitter keyword: Intestinal organoids Hypoxia-reoxygenation Proteomics

Kaatje Lenaerts
contact affiliation	Department of Surgery, Maastricht University, Maastricht
contact email	kaatje.lenaerts@maastrichtuniversity.nl
lab head
Anna M Kip
contact affiliation	Maastricht University
contact email	m.kip@maastrichtuniversity.nl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022999
     1. Label: PRIDE project
     2. Name: Proteomics analysis of human intestinal organoids during hypoxia and reoxygenation as a model to study ischemia-reperfusion injury