PXD022987

PXD022987 is an original dataset announced via ProteomeXchange.

Title	The Ubiquitin-Specific Protease USP36 Promotes Small Nucleolar Ribonucleoprotein Group SUMOylation and is Essential for Ribosome Biogenesis
Description	SUMOylation plays a crucial role in regulating diverse cellular processes including ribosome biogenesis. Proteomic analyses and experimental evidence showed that a number of nucleolar proteins involved in ribosome biogenesis are modified by SUMO. However, how these proteins are SUMOylated is less understood. Here, we report that USP36, a nucleolar deubiquitinating enzyme (DUB), promotes nucleolar SUMOylation. Overexpression of USP36 enhances nucleolar SUMOylation, whereas its knockdown or genetic deletion reduces the levels of SUMOylation. USP36 interacts with SUMO2 and Ubc9 and directly mediates SUMOylation in cells and in vitro. We show that USP36 promotes the SUMOylation of the small nucleolar ribonucleoprotein (snoRNP) components Nop58 and Nhp2 in cells and in vitro and their binding to snoRNAs. It also promotes the SUMOylation of snoRNP components Nop56 and DKC1. Functionally, we show that knockdown of USP36 markedly impairs rRNA processing and translation. Thus, USP36 promotes snoRNP group SUMOylation and is critical for ribosome biogenesis and protein translation.
HostingRepository	PRIDE
AnnounceDate	2021-03-29
AnnouncementXML	Submission_2021-03-29_15:32:13.532.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Phillip Wilmarth
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-07 23:29:51	ID requested
⏵ 1	2021-03-29 15:32:13	announced

Dataset with its publication pending

submitter keyword: Deubiquitinating enzyme, nucleolus, Ribosome biogenesis, SUMO, SUMOylation, USP36, Homo sapiens, Spectral Count, Co-IP, LTQ Velos

Mu-Shui Dai
contact affiliation	Department of Molecular & Medical Genetics Oregon Health & Science University
contact email	daim@ohsu.edu
lab head
Phillip Wilmarth
contact affiliation	OHSU
contact email	wilmarth@ohsu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD022987

PRIDE project URI

• PRIDE
  1. PXD022987
     1. Label: PRIDE project
     2. Name: The Ubiquitin-Specific Protease USP36 Promotes Small Nucleolar Ribonucleoprotein Group SUMOylation and is Essential for Ribosome Biogenesis