PXD022983

PXD022983 is an original dataset announced via ProteomeXchange.

Title	Longitudinal landscape of urinary EV proteome reveals stable protein expression patterns within and between individuals
Description	Urinary extracellular vesicles (EVs) are an attractive source for the detection of disease biomarkers. Mass spectrometry (MS) based proteomics is increasingly used for urinary EV proteome profiling and protein biomarker discovery. However, little is known about the consistency and stability of urinary EV proteins within individuals over time and between different individuals. In this study we profiled the urinary EV proteome of 8 healthy individuals over 6 months at 9 timepoints (total 72 samples) using DIA-MS. We identified a total of 1802 proteins with high correlation amongst all samples. While a minor fraction (10%) of the total urinary EV proteome was shown to play a role in determining personal expression patterns, most of the urinary EV proteins was found to be stable with >90% of the proteins detected in more than 1 person at all timepoints. The variability of urinary EV proteome between different individuals was shown to be significantly higher than personal variation as a result of a small subset (<20%) of proteins. The core interaction network of proteins identified in all individuals revealed EV proteins involved in signaling and localization and sub-clusters enriched for glycolytic activity, protein synthesis, and immune function. Gender-specific expression patterns were detected in the urinary EV proteome, mostly related to hormonal and reproduction pathways. In summary, our findings indicate that the urinary EV proteome is stable in longitudinal samples of healthy subjects, underscoring its potential as a reliable source for protein biomarkers.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_15:29:20.704.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sander Piersma
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-07 23:22:20	ID requested
⏵ 1	2021-09-09 15:29:21	announced

Erozenci LA, Piersma SR, Pham TV, Bijnsdorp IV, Jimenez CR, Longitudinal stability of urinary extracellular vesicle protein patterns within and between individuals. Sci Rep, 11(1):15629(2021) [pubmed]

submitter keyword: Urine, extracellular vesicle, DIA-MS, variability, stability

Connie Ramona Jimenez
contact affiliation	Amsterdam UMC, Vrije Universiteit Amsterdam, Medical Oncology, Cancer Center Amsterdam, OncoProteomics Laboratory, Amsterdam, Netherlands
contact email	c.jimenez@vumc.nl
lab head
Sander Piersma
contact affiliation	Amsterdam UMC
contact email	s.piersma@amsterdamumc.nl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022983
     1. Label: PRIDE project
     2. Name: Longitudinal landscape of urinary EV proteome reveals stable protein expression patterns within and between individuals