<<< Full experiment listing


PXD022980 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIgG M-protein quantification by PRM in blood of multiple myeloma patient
DescriptionThe amino acid sequence of the M-protein for multiple myeloma is unique compared to the polyclonal antibodies in patients’ blood. This uniqueness is exploited to develop an ultra-sensitive M-protein detection method utilizing mass spectrometry (MS). The method involves the de novo amino acid sequencing of the full-length M-protein, and a targeted MS/MS assay to detect and quantify the unique M-protein sequence in serum samples. Healthy control serum spiked with NISTmAb, and serial samples from an MM patient are used to demonstrate the ability of the platform to sequence and monitor a target M-protein. The de novo NISTmAb protein sequence obtained matched the published sequence, confirming the ability of the platform to accurately sequence a target M-protein in serum. NISTmAb was quantified down to 0.0002 g/dL in serum, hundreds of times more sensitive than conventional blood-based tests such as SPEP and IFE. The M-protein in the patient sample could be quantified throughout complete remission, demonstrating the utility of the assay to track M-protein considerably beyond the sensitivities of current blood-based tests. Notably, the assay detected molecular relapse 10 months before detection by IFE. The MS-based assay is highly sensitive, non-invasive and requires only a small amount of serum, less than 100 μl, to monitor M-protein levels in MM patients.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterZac Mc Donald
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListGlu->pyro-Glu; Acetyl; Gln->pyro-Glu; Carboxymethyl; Carbamidomethyl; Oxidation; Label:13C(6)15N(2); Label:13C(6)15N(4)
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-12-07 10:38:24ID requested
12021-07-26 17:00:09announced
Publication List
McDonald Z, Taylor P, Liyasova M, Liu Q, Ma B, Mass Spectrometry Provides a Highly Sensitive Noninvasive Means of Sequencing and Tracking M-Protein in the Blood of Multiple Myeloma Patients. J Proteome Res, 20(8):4176-4185(2021) [pubmed]
Keyword List
submitter keyword: multiple myeloma, PRM, clinical proteomics
Contact List
Bin Ma
contact affiliationUniversity of Waterloo
contact emailbinma@uwaterloo.ca
lab head
Zac Mc Donald
contact affiliationRapid Novor Inc
contact emailzmcdonald@rapidnovor.com
dataset submitter
Full Dataset Link List
Panorama Public dataset URI