PXD022980

PXD022980 is an original dataset announced via ProteomeXchange.

Title	IgG M-protein quantification by PRM in blood of multiple myeloma patient
Description	The amino acid sequence of the M-protein for multiple myeloma is unique compared to the polyclonal antibodies in patients’ blood. This uniqueness is exploited to develop an ultra-sensitive M-protein detection method utilizing mass spectrometry (MS). The method involves the de novo amino acid sequencing of the full-length M-protein, and a targeted MS/MS assay to detect and quantify the unique M-protein sequence in serum samples. Healthy control serum spiked with NISTmAb, and serial samples from an MM patient are used to demonstrate the ability of the platform to sequence and monitor a target M-protein. The de novo NISTmAb protein sequence obtained matched the published sequence, confirming the ability of the platform to accurately sequence a target M-protein in serum. NISTmAb was quantified down to 0.0002 g/dL in serum, hundreds of times more sensitive than conventional blood-based tests such as SPEP and IFE. The M-protein in the patient sample could be quantified throughout complete remission, demonstrating the utility of the assay to track M-protein considerably beyond the sensitivities of current blood-based tests. Notably, the assay detected molecular relapse 10 months before detection by IFE. The MS-based assay is highly sensitive, non-invasive and requires only a small amount of serum, less than 100 μl, to monitor M-protein levels in MM patients.
HostingRepository	PanoramaPublic
AnnounceDate	2021-07-26
AnnouncementXML	Submission_2021-07-26_17:00:08.418.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Zac Mc Donald
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	Glu->pyro-Glu; Acetyl; Gln->pyro-Glu; Carboxymethyl; Carbamidomethyl; Oxidation; Label:13C(6)15N(2); Label:13C(6)15N(4)
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-07 10:38:24	ID requested
⏵ 1	2021-07-26 17:00:09	announced

McDonald Z, Taylor P, Liyasova M, Liu Q, Ma B, Mass Spectrometry Provides a Highly Sensitive Noninvasive Means of Sequencing and Tracking M-Protein in the Blood of Multiple Myeloma Patients. J Proteome Res, 20(8):4176-4185(2021) [pubmed]

submitter keyword: multiple myeloma, PRM, clinical proteomics

Bin Ma
contact affiliation	University of Waterloo
contact email	binma@uwaterloo.ca
lab head
Zac Mc Donald
contact affiliation	Rapid Novor Inc
contact email	zmcdonald@rapidnovor.com
dataset submitter

Panorama Public dataset URI