PXD022970

PXD022970 is an original dataset announced via ProteomeXchange.

Title	PLK1 phosphorylation of Epstein-Barr virus nuclear antigen 2
Description	The Epstein-Barr virus nuclear antigen 2 (EBNA2) initiates and maintains the proliferation of infected B cells. In search of additional cellular strategies, that control EBNA2 function, we have performed a label-free mass spectrometry-based quantification of cellular proteins in EBNA2 immuno-precipitates and found polo-like kinase 1 (PLK1) to be bound to EBNA2. EBNA2/PLK1 complex formation is strongly enforced by EBNA2 S379 phosphorylation catalyzed by the mitotic CYCLIN B/CDK1 complex.
HostingRepository	PRIDE
AnnounceDate	2021-08-22
AnnouncementXML	Submission_2021-10-14_01:05:02.117.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Piero Giansanti
SpeciesList	scientific name: Human herpesvirus 4 strain B95-8; NCBI TaxID: 10377;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-07 03:55:21	ID requested
1	2021-08-22 06:01:33	announced
⏵ 2	2021-10-14 01:05:02	announced	2021-10-14: Updated publication reference for PubMed record(s): 34605140.

Zhang X, Schuhmachers P, Mour, ã, o A, Giansanti P, Murer A, Thumann S, Kuklik-Roos C, Beer S, Hauck SM, Hammerschmidt W, K, ü, ppers R, Kuster B, Raab M, Strebhardt K, Sattler M, M, ü, nz C, Kempkes B, PLK1-dependent phosphorylation restrains EBNA2 activity and lymphomagenesis in EBV-infected mice. EMBO Rep, 22(12):e53007(2021) [pubmed]

submitter keyword: Phosphoproteomics, mass spectrometry, cancer

Bernhard Kuster
contact affiliation	Chair of Proteomics and Bioanalytics, Technical University of Munich, Emil-Erlenmeyer-Forum 5, D-85354, Freising, Germany Bavarian Center for Biomolecular Mass Spectrometry, Technical University of Munich, Gregor-Mendel-Strasse 4, D-85354, Freising, Germany
contact email	kuster@tum.de
lab head
Piero Giansanti
contact affiliation	Technical University of Munich
contact email	piero.giansanti@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/08/PXD022970

PRIDE project URI

• PRIDE
  1. PXD022970
     1. Label: PRIDE project
     2. Name: PLK1 phosphorylation of Epstein-Barr virus nuclear antigen 2