PXD022961

PXD022961 is an original dataset announced via ProteomeXchange.

Title	Lineage‐Mismatched Mitochondrial Replacement in an Inducible Mitochondrial Depletion Model Effectively Restores the Original Proteomic Landscape of Recipient Cells
Description	The therapeutic potential of mitochondrial transfer has been recently demonstrated in a number of preclinical and clinical studies. However, the molecular mechanisms underpinning the success of mitochondrial transfer, including how exogenous mitochondria may influence the cellular proteomic landscape, are largely unknown. Using a mitochondrial depletion model designed to eliminate endogenous cellular mitochondria, we evaluated proteomic profiles in cells following exogenous mitochondrial transplantation. Our data demonstrate that exogenous mitochondrial transfer does not significantly alter the proteomic profile in non-depleted host cells, and largely rescues the aberrant proteome of mitochondrial-depleted cells following internalization. Our data add to a growing body of supportive evidence suggesting that transfer of exogenous mitochondria does not disrupt or perturb the intracellular landscape, and further demonstrate that mitochondrial transfer is capable of restoring cellular phenotype in an induced-depletion model.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:48:24.853.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fausto Capelluto
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-07 03:10:14	ID requested
1	2023-06-15 13:15:56	announced
2	2023-11-14 09:00:58	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:48:25	announced	2024-10-22: Updated project metadata.

10.1002/adbi.202200246;

Capelluto F, Alberico H, Ledo-Hopgood P, Tilly JL, Woods DC, Lineage-Mismatched Mitochondrial Replacement in an Inducible Mitochondrial Depletion Model Effectively Restores the Original Proteomic Landscape of Recipient Cells. Adv Biol (Weinh), 7(6):e2200246(2023) [pubmed]

ProteomeXchange project tag: Human Proteome Project, Mitochondria (HPP)

submitter keyword: mitochondrial depletion

mitochondrial transfer

quantitative proteomics.

Dori C. Woods
contact affiliation	Northeastern University Biology Department
contact email	d.woods@northeastern.edu
lab head
Fausto Capelluto
contact affiliation	Northeastern University
contact email	capelluto.f@northeastern.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD022961

PRIDE project URI

• PRIDE
  1. PXD022961
     1. Label: PRIDE project
     2. Name: Lineage‐Mismatched Mitochondrial Replacement in an Inducible Mitochondrial Depletion Model Effectively Restores the Original Proteomic Landscape of Recipient Cells