PXD022959

PXD022959 is an original dataset announced via ProteomeXchange.

Title	HTPS screen of coagulation proteases at 20C with NaCl, ChCl, LiCl
Description	Goal of the experiment is the characterization of proteases activity and specificity upon Na+ binding. Na+ is an allosteric binders that can induce a conformational change of the protease AS to regulate activity and specificity; only proteases with Y and F in position 225 can coordinate a Na+ molecule.
HostingRepository	PRIDE
AnnounceDate	2021-02-04
AnnouncementXML	Submission_2021-04-06_02:44:12.133.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Federico Uliana
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-07 03:09:59	ID requested
1	2021-02-04 11:29:28	announced
⏵ 2	2021-04-06 02:44:12	announced	2021-04-06: Updated publication reference for PubMed record(s): 33727531.

Uliana F, Vizovi, š, ek M, Acquasaliente L, Ciuffa R, Fossati A, Frommelt F, Goetze S, Wollscheid B, Gstaiger M, De Filippis V, Auf dem Keller U, Aebersold R, Mapping specificity, cleavage entropy, allosteric changes and substrates of blood proteases in a high-throughput screen. Nat Commun, 12(1):1693(2021) [pubmed]

submitter keyword: Protease investigation

Ruedi Aebersold
contact affiliation	Department of Biology, Institute of Molecular Systems Biology, ETH Zürich, Zürich, Switzerland Faculty of Science, University of Zürich, Zürich, Switzerland
contact email	aebersold@imsb.biol.ethz.ch
lab head
Federico Uliana
contact affiliation	ETH
contact email	uliana@imsb.biol.ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD022959

PRIDE project URI

• PRIDE
  1. PXD022959
     1. Label: PRIDE project
     2. Name: HTPS screen of coagulation proteases at 20C with NaCl, ChCl, LiCl