PXD022955

PXD022955 is an original dataset announced via ProteomeXchange.

Title	The proteolytically inactive orthocaspase of Synechocystis sp. PCC 6803 plays an important role during oxidative stress
Description	Caspases are proteases, best known for their involvement in the execution of apoptosis – a subtype of programmed cell death, which occurs only in animals. These proteases are composed of two structural building blocks: a proteolytically active p20 domain and a regulatory p10 domain. Although structural homologues appear in representatives of all other organisms, their functional homology, i. e. cell death depending on their proteolytical activity, is still much disputed. Additionally, pseudo-caspases and pseudo-metacaspases, in which the catalytic histidine-cysteine diad is substituted with non-proteolytic amino acid residues, were shown to be involved in cell death programmes. Here, we present the involvement of a pseudo-orthocaspase (SyOC), a prokaryotic caspase-homologue lacking the p10 domain, in oxidative stress in the model cyanobacterium Synechocystis sp. PCC 6803. To study the in vivo impact of this pseudo-protease during oxidative stress its gene expression during exposure to H2O2 was monitored by RT-qPCR. Furthermore, a knock-out mutant lacking the pseudo-orthocaspase gene was designed and its survival and growth rates were compared to wild type cells as well as its proteome. Deletion of SyOC led to cells with higher tolerance towards oxidative stress, the protein therefore might be involved in a pro-death pathway.
HostingRepository	PRIDE
AnnounceDate	2023-05-14
AnnouncementXML	Submission_2023-05-14_14:05:44.905.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	FranziskaVoellmy
SpeciesList	scientific name: Synechocystis sp. PCC 6803; NCBI TaxID: 1148;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-07 03:09:23	ID requested
1	2021-09-09 14:51:19	announced
⏵ 2	2023-05-14 14:05:45	announced	2023-05-14: Updated project metadata.

Lema A S, Klemen, č, i, č M, V, ö, llmy F, Altelaar M, Funk C, sp. PCC 6803 in Attenuating the Effect of Oxidative Stress. Front Microbiol, 12():634366(2021) [pubmed]

ProteomeXchange project tag: EPIC-XS

submitter keyword: programmed cell death,orthocaspases, stress, metacaspases, pseudo-enzymes, cyanobacteria.

ChristianeFunk
contact affiliation	Dept of Chemistry, Umeå University, Umeå, Sweden.
contact email	christiane.funk@umu.se
lab head
FranziskaVoellmy
contact affiliation	Utrecht University
contact email	franziska.voellmy@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022955
     1. Label: PRIDE project
     2. Name: The proteolytically inactive orthocaspase of Synechocystis sp. PCC 6803 plays an important role during oxidative stress