PXD022954

PXD022954 is an original dataset announced via ProteomeXchange.

Title	Dietary restriction transforms the mammalian protein sulfhydrome in a tissue-specific and cystathionine γ-lyase-dependent manner
Description	Hydrogen sulfide (H2S) is a cytoprotective redox-active metabolite that signals through protein persulfidation (R-SSnH). Despite the known importance of persulfidation on relatively few identified proteins, tissue-specific sulfhydrome profiles and their associated functions are not well characterized, specifically under conditions known to modulate H2S production. We hypothesized that dietary restriction (DR), which increases lifespan and can boost H2S production, expands tissue-specific sulfhydromes. Here, we found protein persulfidation was enriched in liver, kidney, muscle, and brain but decreased in heart of young and aged male mice under two forms of DR, with DR promoting persulfidation in numerous metabolic and aging-related pathways. Mice lacking H2S producing enzyme cystathionine γ-lyase (CGL) had overall decreased tissue protein persulfidation and failed to functionally augment sulfhydromes in response to DR. Overall, we defined tissue- and CGL-dependent sulfhydromes and how diet transforms their makeup, underscoring the breadth for DR and H2S to impact biological processes and organismal health.
HostingRepository	PRIDE
AnnounceDate	2021-01-25
AnnouncementXML	Submission_2021-04-06_02:34:18.425.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD022954
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Nazmin Bithi
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	biotinylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-07 03:09:20	ID requested
1	2021-01-24 23:09:33	announced
⏵ 2	2021-04-06 02:34:19	announced	2021-04-06: Updated publication reference for PubMed record(s): 33741971.

Bithi N, Link C, Henderson YO, Kim S, Yang J, Li L, Wang R, Willard B, Hine C, -lyase-dependent manner. Nat Commun, 12(1):1745(2021) [pubmed]

submitter keyword: dietary restriction (DR), cystathionine γ-lyase (CGL, CSE, CTH), hydrogen sulfide (H2S), sulfhydration, persulfidation, sulfhydrome, biotin thiol assay (BTA), cysteine, every other day (EOD) fasting, longevity, redox metabolism

Christopher Hine
contact affiliation	Cleveland Clinic Lerner Research Institute Cardiovascular and Metabolic Sciences
contact email	hinec@ccf.org
lab head
Nazmin Bithi
contact affiliation	Cleveland Clinic Lerner Research Institute
contact email	bithin@ccf.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022954
     1. Label: PRIDE project
     2. Name: Dietary restriction transforms the mammalian protein sulfhydrome in a tissue-specific and cystathionine γ-lyase-dependent manner