PXD022953

PXD022953 is an original dataset announced via ProteomeXchange.

Title	Pseudomonas syringae effector HopZ3 suppresses PTO immune pathway in tomato by acetylation
Description	The plant pathogen Pseudomonas syringae secretes multiple effectors to suppress plant defenses. Some effectors are recognized by plants while others can function to suppress the resulting immune responses. HopZ3 of Psy B728a is an aminotransferase targeting multiple components of both plant immune complexes and Psy effectors, leading to the activation of these complexes. In Arabidopsis, HopZ3 acetylates the host RPM1 complex and Psy effectors AvrRpm1 and AvrB3 s. In P. syringae resistant tomato, the main immune complex includes PRF and PTO. We found that HopZ3 acts as a virulence factor on tomato by suppressing defenses triggered by Psy AvrPto1. HopZ3 interacts with members of the PTO complex and acetylates PTO, AvrPto1, SlRIPK and SlRIN4s in vitro and in planta. Mapping HopZ3-dependent acetylation sites in planta revealed that AvrPto1 is modified on histidine, serine and threonine residues. The most highly acetylated AvrPto1 residues are T91 and S94 in a loop essential for interaction with PTO. HopZ3 also acetylates, in planta, T204 and T199 in the PTO activation loop which is important for kinase activity and recognition of AvrPto1. In agreement with these findings, we showed that acetylation of either AvrPto1 or PTO by HopZ3 reduces their binding in vitro. Mutation of acetylation sites T91A or H125A/H135A in AvrPto1 does not affect PTO binding in vitro but decreases AvrPto1 recognition in tomato in the absence of HopZ3 and increases bacterial growth. We also found decreased in planta phosphorylation in the presence of HopZ3 for some residues in AvrPto1 and PTO involved in immune signaling. Moreover, acetylation of SlRIPK by HopZ3 reduces its activity as well as phosphorylation of HopZ3 and SlRIN4s. Psy modulation of both its own avirulence effectors and their plant targets leads to decreased defenses and increased susceptibility to infection.
HostingRepository	PRIDE
AnnounceDate	2021-10-18
AnnouncementXML	Submission_2021-10-18_09:30:14.212.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD022953
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Donald Wolfgeher
SpeciesList	scientific name: Pseudomonas syringae pv. syringae; NCBI TaxID: 321;
ModificationList	phosphorylated residue; acetylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-07 03:09:07	ID requested
⏵ 1	2021-10-18 09:30:14	announced

Dataset with its publication pending

submitter keyword: Plant effectors, HopZ3, PTO, PTMs, Phosphorylation, Acetylation, Q-Exactive, LC-MSMS

Dr. Jean T. Greenberg
contact affiliation	Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL, 60637, USA
contact email	jgreenbe@uchicago.edu
lab head
Donald Wolfgeher
contact affiliation	University of Chicago
contact email	donw@uchicago.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/10/PXD022953

PRIDE project URI

• PRIDE
  1. PXD022953
     1. Label: PRIDE project
     2. Name: Pseudomonas syringae effector HopZ3 suppresses PTO immune pathway in tomato by acetylation