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PXD022951

PXD022951 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe immunopeptidome of HLA-A*11:01 expressed K562 cell line
DescriptionThe K562 cell line was obtained from ATCC (American Type Culture Collection, Manassas,VA). And the K562 cell line was transduced using a highly efficient retroviral vector coding HLA-A*11:01. The vectors were transfected into a 293T packaging cell line and replication-defective virus supernatants were harvested. After infection of K562 cells with the supernatant, antibody-directed flow cytometry sorting was done to obtain high expression for HLA-A*11:01. Cells were grown in T75 flasks to a density of 1e9 cells before purification of HLA-I peptides for MS experiments.
HostingRepositoryMassIVE
AnnounceDate2021-07-27
AnnouncementXMLSubmission_2021-07-27_23:40:32.620.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterle zhang
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationListOxidation; Acetyl
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-12-07 02:56:17ID requested
12021-07-27 23:40:32announced
Publication List
no publication
Keyword List
submitter keyword: HLA, MHC, immunopeptidome , epitopes
Contact List
Le Zhang
contact affiliationBGI Education Center
contact emailzhangle2@genomics.cn
lab head
Bo Li
contact affiliationBGI-GenoImmune
contact emaillibo@genomics.cn
lab head
le zhang
contact affiliationUniversity of Chinese Academy of Sciences
contact emailzhangle2@genomics.cn
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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