PXD022947

PXD022947 is an original dataset announced via ProteomeXchange.

Title	The E2F4/p130 repressor complex cooperates with oncogenic Np73 to promote cell survival
Description	Tumor suppressor p53 and its related proteins, p63 and p73, can be synthesized as multiple isoforms lacking part of the N- or C-terminal regions. Specifically, high expression of the Np73 isoform is notoriously associated with poor prognosis in cancer. Here, we have performed proteomics analysis of Np73 using as a cellular model human papillomavirus type 38-transformed keratinocytes (38HK) that express high levels of this isoform. We find that Np73 associates with the E2F4/p130 repressor complex through a direct interaction with E2F4. Remarkably, this interaction is favored by the N-terminal truncation of p73 characteristic of Np73 isoforms and is independent of the C-terminal splicing status. We show that the Np73-E2F4/p130 complex is recruited to the promoters of specific genes, thereby enforcing inhibition of their expression both in 38HK and in cancer-derived cell lines. Consistently, silencing of E2F4 in 38HK and in cancer cells resulted in induction of senescence. In conclusion, we have identified and characterized a novel transcriptional regulatory complex that exerts pro-proliferative functions in transformed cells
HostingRepository	PRIDE
AnnounceDate	2023-02-28
AnnouncementXML	Submission_2023-02-28_07:27:29.328.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	LucNegroni
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-07 00:34:43	ID requested
⏵ 1	2023-02-28 07:27:29	announced

Dataset with its publication pending

submitter keyword: cellular transformation, E2F4, Np73, gene expression, protein-protein interactions,p53

KatiaZanier
contact affiliation	Biotechnology and Cell Signaling, UMR 7242, Strasbourg, France
contact email	zanier@unistra.fr
lab head
LucNegroni
contact affiliation	CNRS
contact email	luc.negroni@igbmc.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/02/PXD022947

PRIDE project URI

• PRIDE
  1. PXD022947
     1. Label: PRIDE project
     2. Name: The E2F4/p130 repressor complex cooperates with oncogenic Np73 to promote cell survival