PXD022939

PXD022939 is an original dataset announced via ProteomeXchange.

Title	Antisense RNA technology induces the loss of function of steroidogenic factor 1 leading to abortion of tilapia
Description	As a precise and efficient genetic engineering method, gene editing technology for animals and plants will gradually move towards large-scale applications, and will have a disruptive impact on the breeding of related traits. By using antisense RNA interference technology to interfere with the mRNA expression and protein translation of the SF-1 gene, we have successfully cultivated tilapia with defective gonadal development. The results of the study showed that the weights of male and female fish in the experimental group were significantly higher than those of the control group. The gonadal weight and gonadal index of the experimental group were significantly lower than those of the control group and the negative control group. HE staining showed that the number of spermatogonia in the testis tissue of the experimental group was significantly reduced, the cavity in the seminiferous tubules was significantly enlarged and increased, the number of sperm cells was significantly decreased, and the number of sperm was relatively rare. However, a large number of stage V oocytes accumulated in the ovarian tissue of female fish in the experimental group, and many of them were gray-white. The mRNA and protein expression levels of SF-1 gene in the gonadal tissues were extremely significantly lower than those of the control and negative controls. The combined analysis of transcriptome and proteome showed that Steroid hormone biosynthesis, Arachidonic acid metabolism and Cell adhesion molecules are involved in the regulation of the gonads of male fish after SF-1 gene silencing. However, ECM-receptor interaction, Retinol metabolism, Nicotinate and nicotinamide metabolism and cytochrome P450 may be involved in the regulation of female fish SF-1 gene silencing. SF-1 silencing may cause developmental defects in the external reproductive organs of male and female fishes and inhibit the development of gonadal tissues through different regulatory pathways. This provides data support for the further functional analysis of SF-1 on fish and the treatment of abnormal human SF-1 expression.
HostingRepository	iProX
AnnounceDate	2020-12-07
AnnouncementXML	Submission_2023-11-25_03:36:29.923.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Junn Qiang
SpeciesList	scientific name: Oreochromis niloticus niloticus; NCBI TaxID: 607699; scientific name: Oreochromis aureus x Oreochromis niloticus; NCBI TaxID: 239089;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-06 23:35:01	ID requested
⏵ 1	2023-11-25 03:36:30	announced

Dataset with its publication pending

submitter keyword: Antisense RNA, steroidogenic factor 1, tilapia

Pao Xu
contact affiliation	Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences
contact email	xup@ffrc.cn
lab head
Junn Qiang
contact affiliation	Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences
contact email	qiangjunn@163.com
dataset submitter

iProX dataset URI