PXD022927 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic analysis of human monosomic cell lines, Label Free MS |
Description | Chromosome loss that results in monosomy has detrimental consequences for human cells, but the underlying molecular mechanisms remain enigmatic. Using p53 deficient monosomic cell lines, we found that chromosome loss impairs proliferation and genomic stability. Transcriptome and proteome analysis revealed a partial compensation of the gene dosage changes that mitigates the effects of chromosome loss. Monosomy triggers global gene expression changes that differ from the effects of trisomy. Strikingly, ribosome biogenesis and translation were commonly downregulated in monosomic cells. Polysome profiling and translation analysis suggest that the defects arise due to haploinsufficiency of ribosomal genes. The ensuing ribosome biogenesis stress triggers the p53 pathway and G1 arrest when TP53 is reintroduced into monosomic cells. Accordingly, impaired ribosome biogenesis and p53 inactivation are associated with monosomy in cancer. Our first systematic study of monosomy in human cells demonstrates that haploinsufficiency of ribosomal genes presents a dominant negative phenotype of monosomy. |
HostingRepository | PRIDE |
AnnounceDate | 2021-06-18 |
AnnouncementXML | Submission_2021-06-18_00:00:05.970.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Paul Menges |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-12-04 07:44:40 | ID requested | |
⏵ 1 | 2021-06-18 00:00:06 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: LC-MS/MS, Q Exactive, Label Free Quantification, Aneuploidy |
Contact List
Zuzana Storchova |
contact affiliation | Department of Biology, University Kaiserslautern, Germany. |
contact email | storchova@bio.uni-kl.de |
lab head | |
Paul Menges |
contact affiliation | Technische Universität Kaiserslautern |
contact email | pmenges@bio.uni-kl.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD022927 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD022927
- Label: PRIDE project
- Name: Proteomic analysis of human monosomic cell lines, Label Free MS