PXD022915

PXD022915 is an original dataset announced via ProteomeXchange.

Title	Proteomics analysis of gastric cancer patients with diabetes mellitus
Description	Proteomics is a powerful approach to study the molecular mechanisms of cancer. In this study, we aim to characterize the proteomic profile of gastric cancer (GC) in patients with diabetes mellitus (DM) type 2. Forty GC tissue samples including 19 cases from diabetic patients and 21 cases from individuals without diabetes (control group) were selected for the proteomics analysis. Gastric tissues were processed following the single-pot, solid-phase-enhanced sample preparation approach—SP3 and enzymatic digestion with trypsin. The resulting peptides were analyzed by LC-MS Liquid Chromatography—Mass Spectrometry (LC-MS). The comparison of protein expression levels between GC samples from diabetic and non-diabetic patients was performed by label-free quantification (LFQ). A total of 6599 protein groups were identified in the 40 samples. Thirty-seven proteins were differentially expressed among the two groups, with 16 upregulated and 21 downregulated in the diabetic cohort. Statistical overresentation tests were considered for different annotation sets including the Gene Ontology(GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), Reactome, and Disease functional databases. Upregulated proteins in the GC samples from diabetic patients were particularly enriched in respiratory electron transport and alcohol metabolic biological processes, while downregulated proteins were associated with epithelial cancers, intestinal diseases, and cell–cell junction cellular components. Taken together, these results support the data already obtained by previous studies that associate diabetes with metabolic disorders and diabetes-associated diseases, such as Alzheimer’s and Parkinson’s, and also provide valuable insights into seven GC-associated protein targets, claudin-3, polymeric immunoglobulin receptor protein, cadherin-17, villin-1, transglutaminase-2, desmoglein-2, and mucin-13, which warrant further investigation.
HostingRepository	PRIDE
AnnounceDate	2021-03-18
AnnouncementXML	Submission_2021-03-18_03:21:18.422.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD022915
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Hugo Osorio
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-04 03:03:56	ID requested
1	2021-01-21 23:46:03	announced
2	2021-02-08 22:19:51	announced	2021-02-09: Updated publication reference for PubMed record(s): 33494396.
3	2021-03-17 05:11:53	announced	2021-03-17: Updated project metadata.
⏵ 4	2021-03-18 03:21:19	announced	2021-03-18: Updated project metadata.

Os, ó, rio H, Silva C, Ferreira M, Gullo I, M, á, ximo V, Barros R, Mendon, ç, a F, Oliveira C, Carneiro F, Proteomics Analysis of Gastric Cancer Patients with Diabetes Mellitus. J Clin Med, 10(3):(2021) [pubmed]

submitter keyword: gastric cancer (GC)

diabetes mellitus (DM)

proteomics

label free quantification (LFQ)

Hugo Osorio
contact affiliation	i3S - Institute for Research and Innovation in Health, University of Porto, Portugal
contact email	hosorio@i3s.up.pt
lab head
Hugo Osorio
contact affiliation	i3S - Institute for Research and Innovation in Health, University of Porto
contact email	hosorio@i3s.up.pt
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022915
     1. Label: PRIDE project
     2. Name: Proteomics analysis of gastric cancer patients with diabetes mellitus