PXD022909

PXD022909 is an original dataset announced via ProteomeXchange.

Title	TLR9-activated B cells imprint adoptively transferred CD8+ T cells with potent tumor immunity and persistence
Description	Here we report a new way to reverse the tolerant state of adoptively transferred CD8+ T cells against melanoma through ex vivo expansion with the TLR9 agonist CpG. CpG-generated T cells elicited potent immunity without co-administration of high dose IL-2 or vaccination, which are adjuvants classically required to effectively treat solid tumors. CpG-expanded T cells exhibited an IL-2RhighICOShighCD39low phenotype ex vivo and engrafted robustly in vivo. In culture, B cells were the only cell type essential for imprinting T cells with this phenotype and potent tumor immunity. CpG agonists targeting B cells, but not dendritic cells, generated CD8+ T cell products with remarkable antitumor properties. Purified B cells were sufficient to mediate the CpG-associated changes in T cells. These findings reveal a vital role for B cells in the generation of effective antitumor T cells and have immediate implications for profoundly improving immunotherapy for patients.
HostingRepository	PRIDE
AnnounceDate	2022-02-22
AnnouncementXML	Submission_2022-02-22_04:25:45.983.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jennifer Bethard
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-04 03:03:20	ID requested
⏵ 1	2022-02-22 04:25:48	announced

Smith AS, Knochelmann HM, Wyatt MM, Rangel Rivera GO, Rivera-Reyes AM, Dwyer CJ, Ware MB, Cole AC, Neskey DM, Rubinstein MP, Liu B, Thaxton JE, Bartee E, Paulos CM, T cells with enhanced tumor immunity. J Immunother Cancer, 10(1):(2022) [pubmed]

submitter keyword: T cells Adoptive cell transfer therapy Toll-like receptors melanoma B cells

Chrystal Paulos PhD
contact affiliation	Medical University of South Carolina College of Medicine Department of Microbiology and Immunology Acting Associate Professor, Department of Surgery, Department of Microbiology and Immunology Emory University School of Medicine Director Translational Research for Cutaneous Malignancies Winship Cancer Institute of Emory University
contact email	chrystal.mary.paulos@emory.edu
lab head
Jennifer Bethard
contact affiliation	Medical University of SC
contact email	bethard@musc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD022909

PRIDE project URI

• PRIDE
  1. PXD022909
     1. Label: PRIDE project
     2. Name: TLR9-activated B cells imprint adoptively transferred CD8+ T cells with potent tumor immunity and persistence