PXD022906

PXD022906 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomics for identification of type I interferon response in primary human glioblastoma multiforme cells
Description	Oncolytic virus therapy is a promising direction for cancer treatment. Numerous oncolytic virus strains are under development or examined in clinical trials, there are examples of FDA approved virus vaccines. Although evident progress exists, oncolytic viruses still suffer low efficiency and the mechanisms of their action remain poorly understood. Defects in antiviral mechanisms that include type I interferon (IFN) signaling, contribute to sensitivity of malignant cells to oncolytic viruses, however, this sensitivity significantly differs for different malignant cells. In this project, we present a collection of representative proteomics data for eight primary glioblastoma multiforme (GBM) cell cultures and one culture of normal astrocytes with established sensitivity to type I IFNs that comprehensively characterize the GBM cell responses to type I IFNs. Project consists of three parts: (1) label-free proteomics of primary GBM cultures on Orbitrap Fusion Lumos (a separate dataset); (2) label-free proteomics of primary GBM and normal astrocytes on Orbitrap QExactive HF; (3) label-based proteomics of wild type, IFIT3-deficient and PLSCR1-deficient DBTRG-05MG cells (ATCC) on Q-Exactive Plus.
HostingRepository	PRIDE
AnnounceDate	2022-02-03
AnnouncementXML	Submission_2022-02-03_12:45:56.558.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Elizaveta Solovyeva
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; formylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-04 01:52:23	ID requested
⏵ 1	2022-02-03 12:45:56	announced

Dataset with its publication pending

submitter keyword: Glioblastoma, Proteomics, Interferone response, LC-MS/MS, Oncolytic viruses

Irina A Tarasova
contact affiliation	laboratory of Physical and Chemical Methods for Structure Analysis, V.L. Talrose Institute for Energy Problems of Chemical Physics, N.N. Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences, Moscow, Russia
contact email	iatarasova@yandex.ru
lab head
Elizaveta Solovyeva
contact affiliation	MIPT
contact email	lisavetasol@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD022906

PRIDE project URI

• PRIDE
  1. PXD022906
     1. Label: PRIDE project
     2. Name: Comparative proteomics for identification of type I interferon response in primary human glioblastoma multiforme cells