PXD022901

PXD022901 is an original dataset announced via ProteomeXchange.

Title	Label-free proteomic strategy to compare the proteomic differences of Inner Mongolian cashmere goat during intramuscular fat anabolism and catabolism
Description	Background: Inner Mongolia is a major raw-cashmere-producing province in China, Inner Mongolia cashmere goat harbors three types —Erlangshan, Aerbasi and Alashan. Nearly 700,000 Aerbasi cashmere goats are fed per year, and meat production is nearly 10,000 tons. However, there are no reports on the meat of this goat. To better understand the molecular variations underlying intramuscular fat (IMF) anabolism and catabolism in Inner Mongolian cashmere goat, the proteomic differences between the biceps femoris (BF) and longissimus dorsi (LD) were investigated by label-free strategy. Then, the proteins were verified by western blot analysis as being involved in IMF anabolism and catabolism. Results: The IMF content was significantly higher in the BF than in the LD, suggesting that IMF is accumulated more in the BF or metabolized more in the LD. We performed proteomic analysis of IMF anabolism and catabolism at the proteomic level, and 1209 proteins were identified in the BF (high-IMF) and LD (low-IMF) groups, with 993 and 896 proteins in each group. Among them, 110 were differentially expressed proteins (DEPs). Gene ontology (GO) classification statistics showed that the 110 DEPs were functionally classified into 100 annotation clusters. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that three pathways were related to IMF metabolism and deposition—fatty acid metabolism, fatty acid degradation and fatty acid elongation—and included 7 proteins. Conclusion: GO and KEGG analyses showed that differentially expressed HADHA, HADHB, ACSL1, ACADS, ACAT1 and ACAA2 in the mitochondrion act via fatty acid metabolism, fatty acid degradation and fatty acid elongation to influence the metabolism and synthesis of long-, short- and medium-chain fatty acids and influence IMF anabolism and catabolism. Protein-protein interaction (PPI) network analysis showed that IMF accumulation in different muscle tissues of Aerbasi cashmere goat was affected by not only 5 key enzymes or proteins involved in fatty acid synthesis and metabolism but also 5 DEPs (SUCLG1, SUCLG2, CS, DLST, ACO2) in the TCA cycle. Our results provide new insights into IMF deposition in goat and improve our understanding of the molecular mechanisms underlying IMF anabolism and catabolism.
HostingRepository	iProX
AnnounceDate	2020-12-04
AnnouncementXML	Submission_2024-01-03_20:34:04.544.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuchun Xie
SpeciesList	scientific name: Capra hircus; NCBI TaxID: 9925;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-03 23:03:11	ID requested
⏵ 1	2024-01-03 20:34:04	announced

Dataset with its publication pending

submitter keyword: cashmere goat meat, IMF, anabolism and catabolism

Jinquan Li
contact affiliation	Inner Mongolia Agricultural University
contact email	lijinquan_nd@126.com
lab head
Yuchun Xie
contact affiliation	College of Animal Science, Inner Mongolia Agricultural University
contact email	878603156@qq.com
dataset submitter

iProX dataset URI