PXD022900

PXD022900 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteome of honeybee brain
Description	A. meliffera and A. cerana are western and eastern honeybee species that evolve a wide spectrum of differences in periphery olfactory system and olfactory driven behaviors. However, the knowledge about the central nervous system regulating the olfaction differences between two bee species is largely unknown. Here, an in-depth phosphoproteome of the antennal lobes (ALs) between two bee species was comparied. Totally, 1227 phosphoprotein, 2951 phosphopeptide and 3906 phosphosite were identified, of which 76% phosphoproteins related to cytoskeleton formation and signal transduction were shared between both bee species, indicates that protein phosphorylation plays a fundamental role in ALs glomeruli formation, synaptic plasticity and signal transduction. Furthermore, 15% (191) and 8.4% (107) phosphoproteins were uniquely expressed in ALs of A. mellifera and A. cerana, respectively, they were mainly involved in ion transmembrane transport, affects ion channel activity. Addtionally, mTOR signaling pathway, a central player of protein synthesis and synaptic plasticity, were enriched in ALs, suggests its important role in olfactory processing, learning and memory formation. Notably, the key phosphoproteins of mTOR pathway were up-regulated in A. cerana ALs relative to A. meliffera, suggests that mTOR signaling pathway may driven the difference olfactory learning and memory ability between two bee species via modification of synaptic plasticity. This data provide firstly comprehensive phosphoproteome profile of both honeybee ALs, and it is potentially useful for further analysis of the neuronal mechanism for the olfactory process by phosphorylation.
HostingRepository	iProX
AnnounceDate	2020-12-03
AnnouncementXML	Submission_2020-12-03_19:34:19.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lifeng Meng
SpeciesList	scientific name: Apis mellifera; NCBI TaxID: 7460;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-03 19:05:29	ID requested
⏵ 1	2020-12-03 19:34:20	announced

Dataset with its publication pending

submitter keyword: Phosphoproteome, Honeybee, Brain

Jianke Li
contact affiliation	Institute of Apicultural Research,Chinese Academy of Agricultural Science, Beijing, China
contact email	apislijk@126.com
lab head
Lifeng Meng
contact affiliation	Insititute of Apiculture Reseach, Chinese Academy of Agricultural Science
contact email	menglifeng@caas.cn
dataset submitter

iProX dataset URI