PXD022895

PXD022895 is an original dataset announced via ProteomeXchange.

Title	Adaptations to high pressure of Nautilia sp. strain PV-1, a piezophilic Epsilonproteobacterium isolated from a deep-sea hydrothermal vent
Description	Physiological and gene expression studies of deep-sea bacteria under pressure conditions similar to those experienced in their natural habitat are critical to understand growth kinetics and metabolic adaptations to in situ conditions. The Epslilonproteobacterium, Nautilia sp. strain PV1, was isolated from hydrothermal fluids released from an active deep-sea hydrothermal vent at 9°N on the East Pacific Rise. Using a high pressure/high temperature continuous culture system we established that strain PV-1 has the shortest generation time of all known piezophilic microorganisms and we investigated its protein expression pattern in response to different hydrostatic pressures. Proteomic analyses of strain PV-1 grown at 200 Bars and 5 Bars showed that pressure adaptation is not restricted only to stress response or homeoviscous adaptation, but that it is more diversified and protein specific, with a fine and variegated regulation of enzymes involved even in the same metabolic pathway. As previously reported, proteins synthesis, motility, transport and energy metabolism are all affected by pressure, although to different extents. In strain PV-1, low pressure condition seems to activate the synthesis of phage-related proteins and an overexpression of enzymes involved in central carbon metabolism.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:46:18.702.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	francesco smedile
SpeciesList	scientific name: Nautilia sp. PV-1; NCBI TaxID: 2579250;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-03 04:10:07	ID requested
1	2023-05-10 13:14:49	announced
⏵ 2	2023-11-14 08:46:26	announced	2023-11-14: Updated project metadata.

Smedile F, Foustoukos DI, Patwardhan S, Mullane K, Schlegel I, Adams MW, Schut GJ, Giovannelli D, Vetriani C, Adaptations to high pressure of Nautilia sp. strain PV-1, a piezophilic Campylobacterium (aka Epsilonproteobacterium) isolated from a deep-sea hydrothermal vent. Environ Microbiol, 24(12):6164-6183(2022) [pubmed]

submitter keyword: high-pressure, deep-sea hydrothermal vent, chemostat, chemosynthesis, thermophile,piezophile, Epsilonproteobacteria

francesco smedile
contact affiliation	Institute for Biological Resources and Marine Biotechnology (IRBIM) (CNR), Spianata San Raineri, 86, 98121 Messina, Italy
contact email	francesco.smedile@cnr.it
lab head
francesco smedile
contact affiliation	CNR italy
contact email	francesco.smedile@cnr.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022895
     1. Label: PRIDE project
     2. Name: Adaptations to high pressure of Nautilia sp. strain PV-1, a piezophilic Epsilonproteobacterium isolated from a deep-sea hydrothermal vent