PXD022892

PXD022892 is an original dataset announced via ProteomeXchange.

Title	Spiroplasma eriocheiris restricts Eriocheir sinensis Wnt signaling pathway to promote pothogen infection and cause host tremor
Description	Spiroplasma eriocheiris, has been identified as a novel lethal pathogen of Eriocheir sinensis tremor disease (TD), one neurological disease with typically paroxysmal tremors of the pereiopod. This pathogen infected and multiplied in the hemocytes of E. sinensis as the first target cells, and then follow the blood circulation to infect the crab other tissues. S. eriocheiris infected the nerves tissue is the directly reason of TD. But the pathogenic mechanism of TD was still few known. Firstly, in the current study, the phosphoproteomic changes of E. sinensis thoracic ganglion after S. eriocheiris infection were obtained. KEGG pathway analysis show Wnt signaling pathways was restrained, corresponding many nervous system development and signal transmission pathway also destroyed. Base on the identified modified sequence, several peptides (GSK3β, SYN, VAMP and SNAMP-25) were selected to synthesize chemically and prepare phosphorylated antibodies. The differentially expressed phosphorylated proteins GSK3β-pSer9, VAMP-pSer72, SNAP25-pSer102, and SYN-pSer134 in thoracic ganglion were significantly down-regulated verified by immunohistochemistry and western blot, this results are similar with the phosphoproteomic. By qRT-PCR, western blot, RNA interference and inhibitor experiments, when the S. eriocheiris infected the hemocytes of crab, the GSK-3β and β-Catenin in Wnt-β-Catenin pathway were restrained similar in the thoracic ganglion. The S. eriocheiris can restricts the hemocytes Wnt-β-Catenin pathway to help itself infection no matter in vivo or in vitro. Neurotransmitter metabolite analysis showed that four kinds of neurotransmitters (5-Hydroxy-L-tryptophan, Serotonin, Acetylcholine and γ-Amino-butyric acid) in thoracic ganglion were metabolic disorders in E. sinensis thoracic ganglion after S. eriocheiris infection. The present work could serve as a basis for understanding the role of Wnt signaling pathway in the process of S. eriocheiris invasion E. sinensis hemocytes and causing paroxysmal tremors of E. sinensis the pereiopod.
HostingRepository	PRIDE
AnnounceDate	2025-10-06
AnnouncementXML	Submission_2025-10-05_16:32:48.796.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qingguo Meng
SpeciesList	scientific name: Eriocheir sinensis (Chinese mitten crab); NCBI TaxID: NEWT:95602;
ModificationList	phosphorylated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-03 03:18:01	ID requested
⏵ 1	2025-10-05 16:32:49	announced

Hou L, Yao Y, Ma Y, Gu W, Wang W, Sun W, Meng Q, Activity Facilitates the Infection of Spiroplasma and Leads to Neuropathic Diseases in Crustaceans. Mol Cell Proteomics, 24(9):101059(2025) [pubmed]

10.1016/j.mcpro.2025.101059;

submitter keyword: Wnt signaling pathway

Eriocheir sinensis

Spiroplasma eriocheiris

Tremor disease

thoracic ganglion

Qingguo Meng
contact affiliation	Nanjing Normal University
contact email	mlzzcld@aliyun.com
lab head
Qingguo Meng
contact affiliation	Nanjing Normal University
contact email	mlzzcld@aliyun.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022892
     1. Label: PRIDE project
     2. Name: Spiroplasma eriocheiris restricts Eriocheir sinensis Wnt signaling pathway to promote pothogen infection and cause host tremor