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PXD022891

PXD022891 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAutomated 16plex plasma proteomics with real-time search and ion mobility mass spectrometry enables large scale profiling in naked mole-rats and mice.
DescriptionPerforming large-scale plasma proteome profiling is challenging due to limitations imposed by lengthy preparation and instrument time. We present a fully Automated Multiplexed Proteome Profiling Platform (AutoMP3) using the Hamilton VantageTM liquid handling robot capable of preparing hundreds to thousands of samples. To maximize protein depth in single shot runs we combined 16plex Tandem Mass Tags (TMTpro) with high-field asymmetric waveform ion mobility spectrometry (FAIMS Pro) and real-time search (RTS). We quantified over 40 proteins / min / sample, doubling the previously published rates. We applied AutoMP3 to investigate the naked mole-rat plasma proteome both as a function of circadian cycle and in response to ultraviolet (UV) treatment. In keeping with the lack of synchronized circadian rhythms in naked mole-rats, we find few circadian patterns in plasma proteins over the course of 48hr. Furthermore, we quantify many disparate changes between mice and naked mole-rats at both 48hr and one week after UV exposure. These species differences in plasma protein temporal responses could contribute to the pronounced cancer resistance observed in naked mole-rats.
HostingRepositoryPRIDE
AnnounceDate2021-02-04
AnnouncementXMLSubmission_2021-02-04_00:53:47.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSasha Gaun
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Heterocephalus glaber; NCBI TaxID: 10181;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-12-03 03:17:48ID requested
12021-02-04 00:53:48announced
Publication List
Gaun A, Lewis Hardell KN, Olsson N, O'Brien JJ, Gollapudi S, Smith M, McAlister G, Huguet R, Keyser R, Buffenstein R, McAllister FE, Automated 16-Plex Plasma Proteomics with Real-Time Search and Ion Mobility Mass Spectrometry Enables Large-Scale Profiling in Naked Mole-Rats and Mice. J Proteome Res, 20(2):1280-1295(2021) [pubmed]
Keyword List
submitter keyword: Mouse and Naked-Mole Rat Plasma
Contact List
Fiona E. McAllister
contact affiliationCalico Labs, United States
contact emailfiona@calicolabs.com
lab head
Sasha Gaun
contact affiliationCalico Labs
contact emailaleksandergaun@gmail.com
dataset submitter
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Dataset FTP location
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