PXD022880

PXD022880 is an original dataset announced via ProteomeXchange.

Title	Multi-omics revealed molecular mechanisms underlying guard cell systemic acquired resistance
Description	Systemic Acquired Resistance (SAR) as a plant immune response improves immunity of distal tissue after local exposure to a pathogen. Stomata pores on leaf surfaces are formed by guard cells that recognize bacterial pathogens via pattern recognition receptors. We found that Arabidopsis thaliana plants that have been previously exposed to the pathogenic bacteria Pseudomonas syringae pv. tomato DC3000 (Pst) exhibit an altered stomatal response compared to control plants when systemic leaves are later exposed to the bacteria. Reduced stomatal apertures of SAR primed plants lead to decreases in the number of bacteria that enter the apoplastic space of the leaves. To examine how SAR affects molecular processes in guard cells of distal leaves, we collected Arabidopsis guard cell samples and extracted lipids, metabolites and proteins using a 3-in-1 method. Multi-omic results have revealed molecular components of SAR response specific to the functions of guard cells and roles of ROS and fatty acid signaling in guard cell SAR response. Additionally, our results show an increase in palmitic acid and its derivative 9-PAHSA in primed guard cells. Palmitic acid may play a role as an agonist to Flagellin Sensitive 2 (FLS2), which initiates stomatal closure upon perception of bacterial flagella. The improved understanding of how SAR immune signals affect stomatal movement and immunity can aid biotechnology and marker-based breeding of crops for enhanced disease resistance.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_14:44:16.734.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lisa David
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-02 23:58:00	ID requested
⏵ 1	2021-09-09 14:44:17	announced

David L, Kang J, Dufresne D, Zhu D, Chen S, Multi-Omics Revealed Molecular Mechanisms Underlying Guard Cell Systemic Acquired Resistance. Int J Mol Sci, 22(1):(2020) [pubmed]

submitter keyword: Systemic Acquired Resistance, Guard cell, Immunity, Multi-omics, Redox, Fatty acid

Sixue Chen
contact affiliation	University of Florida Biology Department
contact email	schen@ufl.edu
lab head
Lisa David
contact affiliation	University of Florida
contact email	lisaidavid@ufl.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD022880

PRIDE project URI

• PRIDE
  1. PXD022880
     1. Label: PRIDE project
     2. Name: Multi-omics revealed molecular mechanisms underlying guard cell systemic acquired resistance