PXD022879

PXD022879 is an original dataset announced via ProteomeXchange.

Title	Adaptive responses of Pseudomonas aeruginosa to treatment with antibiotics and non-steroidal anti-inflammatory drugs
Description	Circumventing or overwhelming the bacterial adaptation capabilities is key to combatting multidrug-resistant pathogens like Pseudomonas aeruginosa. We investigated the physiological stress exerted by approved antibiotics (ciprofloxacin, levofloxacin, rifampicin, gentamicin, tobramycin, azithromycin, tigecycline, polymyxin B, colistin, ceftazidime, meropenem, piperacillin/tazobactam), experimental antibiotics (CHIR-090) and NSAIDs (acetylsalicylic acid (aspirin), diclofenac, ibuprofen), and studied the bacterial response on the proteome level. Radioactive pulse-labeling of newly synthesized proteins followed by 2D-PAGE was used to monitor the acute response of P. aeruginosa to antibiotic treatment. Subsequently, marker proteins were excised from non-radioactive gels and identified by mass spectrometry. We generated a reference library of P. aeruginosa proteomic responses and implemented a mathematical comparison of the profiles. Proteomic signatures were derived for clinically relevant target areas.
HostingRepository	PRIDE
AnnounceDate	2022-08-12
AnnouncementXML	Submission_2022-08-12_01:32:19.508.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dominik Wüllner
SpeciesList	scientific name: Pseudomonas aeruginosa PAO1; NCBI TaxID: 208964;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-02 23:02:46	ID requested
⏵ 1	2022-08-12 01:32:20	announced

W, ü, llner D, Gesper M, Haupt A, Liang X, Zhou P, Dietze P, Narberhaus F, Bandow JE, to Treatment with Antibiotics. Antimicrob Agents Chemother, 66(1):e0087821(2022) [pubmed]

submitter keyword: LPS biosynthesis inhibition, mode of action, proteomics, Pseudomonas aeruginosa, stress response

Julia Elisabeth Bandow
contact affiliation	Applied Microbiology, Faculty of Biology and Biotechnology, Ruhr University Bochum, 44780 Bochum, Germany
contact email	julia.bandow@rub.de
lab head
Dominik Wüllner
contact affiliation	Ruhr University Bochum
contact email	Dominik.Wuellner@rub.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD022879

PRIDE project URI

• PRIDE
  1. PXD022879
     1. Label: PRIDE project
     2. Name: Adaptive responses of Pseudomonas aeruginosa to treatment with antibiotics and non-steroidal anti-inflammatory drugs