PXD022864 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic profiling of plasma membrane proteins from bovine rod photoreceptor outer segments |
Description | The outer segment (OS) organelle of vertebrate photoreceptors is a highly specialized cilium whose plasma membrane plays vital and diverse roles in supporting photoreceptor function and health. However, little is known about the identity of its protein constituents, as this membrane cannot be purified to homogeneity. In this study, we employed the technique of protein correlation profiling to identify unique OS plasma membrane proteins. To achieve this, we used label-free quantitative mass spectrometry to compare relative protein abundances in an enriched preparation of the OS plasma membrane to a preparation of total outer segment membranes. We have found that only five proteins were enriched at the same level as previously validated OS plasma membrane markers. Two of these proteins, TMEM67 and TMEM237, had not been previously assigned to this membrane and one, embigin, had not been identified in photoreceptors. We further showed that embigin associates with monocarboxylate transporter MCT1 in the OS plasma membrane, facilitating lactate transport through this cellular compartment. |
HostingRepository | PRIDE |
AnnounceDate | 2021-04-30 |
AnnouncementXML | Submission_2021-05-06_23:11:41.076.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD022864 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Nikolai Skiba |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-12-02 02:46:15 | ID requested | |
1 | 2021-04-30 12:24:11 | announced | |
⏵ 2 | 2021-05-06 23:11:41 | announced | 2021-05-07: Updated publication reference for PubMed record(s): 33933680. |
Publication List
Skiba NP, Cady MA, Molday L, Han JYS, Lewis TR, Spencer WJ, Thompson WJ, Hiles S, Philp NJ, Molday RS, Arshavsky VY, TMEM67, TMEM237, and Embigin in Complex With Monocarboxylate Transporter MCT1 Are Unique Components of the Photoreceptor Outer Segment Plasma Membrane. Mol Cell Proteomics, 20():100088(2021) [pubmed] |
Keyword List
submitter keyword: retina, photoreneptor, outer segment, protein correlation profiling |
Contact List
Vadim Arshavsky |
contact affiliation | Duke University Eye Center |
contact email | vadim.arshavsky@duke.edu |
lab head | |
Nikolai Skiba |
contact affiliation | Duke University |
contact email | nikolai.skiba@duke.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD022864
- Label: PRIDE project
- Name: Proteomic profiling of plasma membrane proteins from bovine rod photoreceptor outer segments