PXD022860 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic analysis of whole brains from Ndufs4-/- mice treated with doxycycline |
Description | Mice with mitochondrial complex I deficiency (Ndufs4-/-) suffer from severe energy impairment primarily affecting the brain and die at P55. A small molecule screen developed by our lab using cells harboring human mitochondrial disease mutations identified antibiotics targeting mitochondrial translation as potent inhibitors of cell death under nutrient stress conditions. Doxycycline, which was identified amongst these antibiotics, was administered in the diet of Ndufs4-/- mice after weaning at P21. Mice fed with doxycycline showed improved motor function and significantly increased lifespan relative to untreated Ndufs4-/- mice. In order to investigate the molecular changes promoted by doxycycline in the brains of these mice, Ndufs4-/- doxycycline treated (DOX), Ndufs4-/- untreated (KO), and Ndufs4+/+ (WT) control mice were sacrificed at P55 and their brains harvested. Proteomic analysis revealed doxycycline treatment largely prevented the neuroimmune and inflammatory profile identified in the Ndufs4-/- mice. These findings implicate a potentially causality of these proteins in the neuronal cell death ultimately leading to the observed brain pathology. |
HostingRepository | PRIDE |
AnnounceDate | 2021-09-09 |
AnnouncementXML | Submission_2021-09-09_14:40:59.441.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | mark Jedrychowski |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-12-01 23:43:02 | ID requested | |
⏵ 1 | 2021-09-09 14:41:00 | announced | |
Publication List
Perry EA, Bennett CF, Luo C, Balsa E, Jedrychowski M, O'Malley KE, Latorre-Muro P, Ladley RP, Reda K, Wright PM, Gygi SP, Myers AG, Puigserver P, Tetracyclines promote survival and fitness in mitochondrial disease models. Nat Metab, 3(1):33-42(2021) [pubmed] |
Keyword List
submitter keyword: Mitochondrial disease, Tetracycline, Mitochondrial translation, Mouse brain |
Contact List
Pere Puigserver |
contact affiliation | Dana Farber Cancer Institute Harvard Medical School Boston, Ma |
contact email | Pere_Puigserver@dfci.harvard.edu |
lab head | |
mark Jedrychowski |
contact affiliation | Harvard Medical School |
contact email | mark_jedrychowski@hms.harvard.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD022860 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD022860
- Label: PRIDE project
- Name: Proteomic analysis of whole brains from Ndufs4-/- mice treated with doxycycline