PXD022857

PXD022857 is an original dataset announced via ProteomeXchange.

Title	O-GlcNAc sites identification and quantification proteomics analysis of O-GlcNAc chromatin in MCF-7 and MCF-7/ADR cells
Description	We used a metabolic labeling (GalNAz) based O-GlcNAc sites identification and quantification proteomics method to compare the O-GlcNAc chromatin of MCF-7 and MCF-7/ADR cells. The O-GlcNAc chromatin-associated proteins can be metabolically labeled with azides, followed by reaction with a cleavable DADPS (dialkoxydiphenylsilane) alkyne-biotin linker and enrichment with streptavidin beads. After cleavage of DADPS by formic acid, O-GlcNAc modified peptides were quantitative analysis using tandem mass spectroscopy (MS).
HostingRepository	iProX
AnnounceDate	2020-12-01
AnnouncementXML	Submission_2020-12-02_18:46:41.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yubo Liu
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-01 23:18:10	ID requested
⏵ 1	2020-12-02 18:46:42	announced

Dataset with its publication pending

submitter keyword: O-GlcNAc, metabolic labeling, proteomics

Yubo Liu
contact affiliation	Dalian University of technology
contact email	liuyubo@dlut.edu.cn
lab head
Yubo Liu
contact affiliation	Dalian University of technology
contact email	liuyubo@dlut.edu.cn
dataset submitter

iProX dataset URI