PXD022853

PXD022853 is an original dataset announced via ProteomeXchange.

Title	NHE1 phosphorylation analysis in cardiomyocytes in normoxic and anoxic conditions
Description	Na+/H+ exchanger 1 (NHE1; SLC9A1) is a transport protein responsible for pH regulation in various types of cells. In cardiac myocytes, NHE1 generates the largest transmembrane acid-extrusion flux and is therefore the most significant controller of the intracellular acid/base milieu. The protein itself is regulated by various enzymes, including kinases. A number of critically important sites have been identified, coupling NHE1 to signalling cascades such as cAMP. NHE1 activity is very sensitive to oxygen tension, thus linking metabolism with pH. However, the sites implicated in this effect are not known. This study identified novel phosphorylation sites on NHE1. The state of these residues regulates NHE1 activity, hence pH and a myriad of pH sensitive downstream processes. Dysregulated NHE1 activity has been shown in diseases of the heart. Identification of novel regulatory sites can help demarcate the mechanisms of disease and suggest possible interventions to correct NHE1 activity.
HostingRepository	PRIDE
AnnounceDate	2021-01-26
AnnouncementXML	Submission_2021-01-25_22:34:33.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marjorie Fournier
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-01 08:44:09	ID requested
⏵ 1	2021-01-25 22:34:34	announced

Dataset with its publication pending

submitter keyword: Na+/H+ exchanger 1, anoxic, phosphorylation

Pawel Swietach
contact affiliation	University of Oxford, Department of Physiology, Anatomy and Genetics
contact email	pawel.swietach@dpag.ox.ac.uk
lab head
Marjorie Fournier
contact affiliation	University of Oxford
contact email	marjorie.fournier@bioch.ox.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022853
     1. Label: PRIDE project
     2. Name: NHE1 phosphorylation analysis in cardiomyocytes in normoxic and anoxic conditions