PXD022842

PXD022842 is an original dataset announced via ProteomeXchange.

Title	Patagonin - CRISP: Antimicrobial activity and source of antimicrobial molecules in Duvernoy's gland secretion - Philodryas patagoniensis snake
Description	Snake venoms contain a variety of toxins with a range of biological activity. Among these are the cysteine-rich secreted proteins (CRISPs). The proteins of this family have masses of 20–30 kDa and display homologous amino acid sequences containing 16 cysteine residues, forming eight disulfide bonds. Some of these proteins have been explored, characterized, and described in terms of their activity; however, little is known of their range of activities. A search for new antimicrobial molecules is ongoing, as the number of microbial strains resistant to available antibiotics is increasing. We identified antimicrobial activity in the secretion of the Duvernoy’s gland of the rear-fanged Philodryas patagoniensis. Fractions of this venom were subjected to Reverse Phase – High Performance Liquid Chromatography and analyzed to determine their antimicrobial activity with a liquid broth inhibition assay. One of the fractions presented activity against a Gram-negative bacterium and a filamentous fungus. This fraction was analyzed with LC-MS/MS, and a protein of 24,848.8 Da was identified. Database searches allowed us to identify it as a CRISP due to the presence of some unique fragments in the molecule. We called it patagonin-CRISP, as the same protein in the venom of P. patagoniensis had previously been characterized as having a different biological activity. Patagonin-CRISP presented activity at very low concentrations and showed no cytotoxic activity. This is the first time that antimicrobial activity has been identified for P. patagoniensis venom or for a CRISP family protein.
HostingRepository	PRIDE
AnnounceDate	2021-02-01
AnnouncementXML	Submission_2021-01-31_22:16:35.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Juliana Badari
SpeciesList	scientific name: Philodryas patagoniensis; NCBI TaxID: 120310;
ModificationList	monohydroxylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-01 02:26:21	ID requested
⏵ 1	2021-01-31 22:16:36	announced

Dataset with its publication pending

submitter keyword: CRISP

Antimicrobials

Bioprospecting

Dipsadidae

Snakes

Rear-fanged.

Pedro Ismael da Silva Junior
contact affiliation	Butantan Institute
contact email	pisjr@butantan.gov.br
lab head
Juliana Badari
contact affiliation	Butantan Institute
contact email	juliana.badari@butantan.gov.br
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022842
     1. Label: PRIDE project
     2. Name: Patagonin - CRISP: Antimicrobial activity and source of antimicrobial molecules in Duvernoy's gland secretion - Philodryas patagoniensis snake