PXD022834

PXD022834 is an original dataset announced via ProteomeXchange.

Title	Comparative cell surface proteomic analysis of the primary human T cell and monocyte responses to type I interferon
Description	The cellular response to interferon (IFN) is essential for antiviral immunity, IFN-based therapy and IFN-related disease. The plasma membrane (PM) provides a critical interface between the cell and its environment, and is the initial portal of entry for viruses. Nonetheless, the effect of IFN on PM proteins is surprisingly poorly understood, and has not been systematically investigated in primary immune cells. Here, we use multiplexed proteomics to quantify IFNα-stimulated PM protein changes in primary human CD14+ monocytes and CD4+ T cells from five donors, quantifying 606 and 482 PM proteins respectively. Comparison of cell surface proteomes revealed a remarkable invariance between donors in the overall composition of the cell surface from each cell type, but a marked donor-to-donor variability in the effects of IFNα. Furthermore, whereas only 2.7% of quantified proteins were consistently upregulated by IFNα at the surface of CD4+ T cells, 6.8% of proteins were consistently upregulated in primary monocytes, suggesting that the magnitude of the IFNα response varies according to cell type. Amongst these differentially regulated proteins, we found the viral target Endothelin-converting enzyme 1 (ECE1) to be an IFNα-stimulated protein exclusively upregulated at the surface of CD4+ T cells. We therefore provide a comprehensive map of the cell surface of IFNα-stimulated primary human immune cells, including previously uncharacterised interferon stimulated genes (ISGs) and candidate antiviral factors.
HostingRepository	PRIDE
AnnounceDate	2021-02-09
AnnouncementXML	Submission_2021-02-08_22:25:17.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lior Soday
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-01 02:25:44	ID requested
⏵ 1	2021-02-08 22:25:18	announced

Dataset with its publication pending

submitter keyword: Interferon type I (IFN), leukocytes, quantitative proteomics, cell surface, antiviral restriction, monocyte, T cell, plasma membrane

Michael Weekes
contact affiliation	Cambridge Institute for Medical Research, University of Cambridge, Hills Road, Cambridge, CB2 0XY, UK
contact email	Mpw1001@cam.ac.uk
lab head
Lior Soday
contact affiliation	University of Cambridge
contact email	ls563@cam.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022834
     1. Label: PRIDE project
     2. Name: Comparative cell surface proteomic analysis of the primary human T cell and monocyte responses to type I interferon