PXD022833

PXD022833 is an original dataset announced via ProteomeXchange.

Title	Discovering the N-terminal Methylome by the Repurposing of Proteomic Datasets
Description	Protein ⍺-N-methylation is a cryptic and relatively unexplored post-translational modification involving the covalent addition of methyl groups to the free a-amino group at protein N-termini. To systematically explore the extent of ⍺-N-terminal methylation in yeast and humans, we utilized a repurposing method optimized for ⍺-N-terminome identification to take advantage of publicly accessible proteomic datasets. Searching of four datasets indicated the ⍺-N-methylation of established and novel substrates with canonical N-terminal motifs of established ⍺-N-terminal methyltransferases, NTMT1/2 for humans and Tae1 for yeast. Moreover, ⍺-N-methylation of non-canonical sequences was more prevalent than expected, suggesting unappreciated and cryptic methylation events. Analysis of the amino acid frequencies of ⍺-methylated peptides revealed enrichment of serine, alanine, proline and glycine at the first position for both yeast and human, suggesting amino acid sequence conservation is not required for some types of ⍺-methylation events. We delineated numerous amino acids in which there is evidence of competition between acetylation and methylation. We tested three potentially methylated proteins and confirmed the ⍺-N-terminal methylation of Hsp31. The other two proteins, Vma1 and Ssa3, were found to be predominantly acetylated. This study demonstrates the feasibility of reprocessing proteomic data for global ⍺-N-terminal methylome investigations.
HostingRepository	PRIDE
AnnounceDate	2021-06-25
AnnouncementXML	Submission_2021-08-13_00:47:34.267.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD022833
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Panyue Chen
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	alpha-amino acetylated residue; alpha-amino monomethylated residue; alpha-amino trimethylated residue; alpha-amino dimethylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-01 02:25:30	ID requested
1	2021-03-25 07:16:50	announced
⏵ 2	2021-08-13 00:47:35	announced	2021-08-13: Updated publication reference for PubMed record(s): 34382793.

Chen P, Paschoal Sobreira TJ, Hall MC, Hazbun TR, Discovering the N-Terminal Methylome by Repurposing of Proteomic Datasets. J Proteome Res, 20(9):4231-4247(2021) [pubmed]

submitter keyword: ⍺-N-terminal methylation, proteomics, repurposing , post-translational modification

Tony Hazbun
contact affiliation	Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN47907
contact email	thazbun@purdue.edu
lab head
Panyue Chen
contact affiliation	Purdue University
contact email	cpanyue@purdue.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022833
     1. Label: PRIDE project
     2. Name: Discovering the N-terminal Methylome by the Repurposing of Proteomic Datasets