PXD022828
PXD022828 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | The neomycin resistance cassette in the targeted allele of Shank3B knock-out mice has potential off-target effects to produce an unusual Shank3 isoform |
| Description | Variants of the SH3 and multiple ankyrin repeat domains 3 (SHANK3), which encodes postsynaptic scaffolds, are associated with brain disorders. The targeted alleles in a few Shank3 knock-out (KO) lines contain a neomycin resistance (Neo) cassette, which may perturb the normal expression of neighboring genes; however, this has not been investigated in detail. We previously reported an unexpected increase in the mRNA expression of Shank3 exons 1~12 in the brains of Shank3B KO mice generated by replacing Shank3 exons 13~16 with the Neo cassette. In this study, we confirmed that the increased Shank3 mRNA in Shank3B KO brains produced an unusual ~60 kDa Shank3 isoform (Shank3-N), which did not properly localize to the synaptic compartment. |
| HostingRepository | MassIVE |
| AnnounceDate | 2021-01-07 |
| AnnouncementXML | Submission_2021-01-07_17:03:18.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Heeyoun Hwang |
| SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
| ModificationList | L-methionine (R)-sulfoxide; iodoacetamide - site C |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2020-11-30 23:48:41 | ID requested | |
| ⏵ 1 | 2021-01-07 17:03:18 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: shank3, neomycin |
Contact List
| Jin Young Kim | |
|---|---|
| contact affiliation | Korea Basic Science Institute |
| contact email | jinyoung@kbsi.re.kr |
| lab head | |
| Heeyoun Hwang | |
| contact affiliation | KBSI |
| contact email | heeyounh@kbsi.re.kr |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000086539/ |
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