PXD022807

PXD022807 is an original dataset announced via ProteomeXchange.

Title	Flow Cytometry Analysis of Circulating Extracellular Vesicle Subtypes from Fresh Body Fluids
Description	Extracellular vesicles (EVs) are released by shedding during different physiological processes and are increasingly thought to be new potential biomarkers. However, the impact of pre-analytical processing phases on the final measurement is not predictable and, for this reason, the translation of basic research into clinical practice has been precluded. Here we have optimized a simple procedure in combination with PFC, to identify, classify, enumerate, and separate circulating EVs from different cell origins. This protocol takes advantage of a lipophilic cationic dye (LCD) able to probe EVs. Moreover, the application of the newly optimized PFC protocol here described allowed the obtainment of repeatable EV counts. The translation of this PFC protocol to fluorescence-activated cell sorting allowed us to separate EVs from fresh peripheral blood samples. Sorted EV preparations resulted particularly suitable for proteomic analyses, which we applied to study their protein cargo. Here we show that LCD staining allowed PFC detection and sorting of EVs from fresh body fluids, avoiding pre-analytical steps of enrichment that could impact final results. Therefore, LCD staining is an essential step towards the assessment of EV clinical significance.
HostingRepository	PRIDE
AnnounceDate	2021-01-04
AnnouncementXML	Submission_2021-01-04_06:07:40.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Piero Del Boccio
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-30 03:58:23	ID requested
⏵ 1	2021-01-04 06:07:41	announced

Marchisio M, Simeone P, Bologna G, Ercolino E, Pierdomenico L, Pieragostino D, Ventrella A, Antonini F, Del Zotto G, Vergara D, Celia C, Di Marzio L, Del Boccio P, Fontana A, Bosco D, Miscia S, Lanuti P, Flow Cytometry Analysis of Circulating Extracellular Vesicle Subtypes from Fresh Peripheral Blood Samples. Int J Mol Sci, 22(1):(2020) [pubmed]

submitter keyword: Extracellular Vesicles

Polychromatic Flow Cytometry

Biomarkers

Proteomics

Fresh Peripheral Blood.

Piero Del Boccio
contact affiliation	Center for Advanced Studies and Technology, "G. d'Annunzio" University, Chieti, Italy
contact email	piero.delboccio@unich.it
lab head
Piero Del Boccio
contact affiliation	University "G. d'Annunzio" of Chieti-Pescara
contact email	piero.delboccio@unich.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD022807

PRIDE project URI

• PRIDE
  1. PXD022807
     1. Label: PRIDE project
     2. Name: Flow Cytometry Analysis of Circulating Extracellular Vesicle Subtypes from Fresh Body Fluids