PXD022799

PXD022799 is an original dataset announced via ProteomeXchange.

Title	Long noncoding RNA lnc-POP1-1 upregulated by VN1R5 promotes cisplatin resistance in head and neck squamous cell carcinoma through interaction with MCM5
Description	Cisplatin resistance is a major therapeutic challenge in advanced head and neck squamous cell carcinoma (HNSCC). Here, we aimed to investigate the key signaling pathway for cisplatin resistance in HNSCC cells. HNSCC cell lines that were sensitive (HN4 and HN30) or resistant (HN4/DDP and HN30/DDP) to cisplatin were used for this study. Moreover, the cisplatin-resistant human melanoma cell lines (A375/DDP) and human lung cancer cell lines (A549/DDP) have also been established. To identify the role of proteins in the acquisition of cisplatin resistance, we analyzed the abnormally expressed protein via protein mass spectrometry methods (isobaric tags for relative and absolute quantitation, iTRAQ) in cisplatin-sensitive and cisplatin-resistant cancer cells, and found that VN1R5 was highly expressed in cisplatin-resistant cells. Long noncoding RNA lnc-POP1-1 upregulated by VN1R5. To deeply investigate the mechanism by which lnc-POP1-1 affects cisplatin resistance in HNSCC cells, we used RNA pull-down assays followed by mass spectrometry to explore the putative RNA-binding proteins (RBPs) interacting with lnc-POP1-1.
HostingRepository	iProX
AnnounceDate	2020-11-28
AnnouncementXML	Submission_2023-02-20_19:23:07.683.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yingying Jiang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive; Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-29 17:27:35	ID requested
1	2020-11-29 17:29:50	announced
⏵ 2	2023-02-20 19:23:12	announced	2023-02-21: Update information.

Jiang Y, Guo H, Tong T, Xie F, Qin X, Wang X, Chen W, Zhang J, lncRNA lnc-POP1-1 upregulated by VN1R5 promotes cisplatin resistance in head and neck squamous cell carcinoma through interaction with MCM5. Mol Ther, 30(1):448-467(2022) [pubmed]

submitter keyword: Long noncoding RNA, lnc-POP1-1, VN1R5, MCM5, Cisplatin resistance, DNA repair, Head and neck squamous cell carcinoma

Jianjun Zhang
contact affiliation	Shanghai Jiao Tong University School of Medicine
contact email	zjjshuobo@163.com
lab head
Yingying Jiang
contact affiliation	Shanghai Jiao Tong University School of Medicine
contact email	yingying820814@163.com
dataset submitter

iProX dataset URI