PXD022769

PXD022769 is an original dataset announced via ProteomeXchange.

Title	Identification of a lipid droplet-plasma membrane tethering complex
Description	Membrane contact sites (MCS) are interorganellar contacts that allow for the direct exchange of molecules such as lipids or Ca2+ between organelles, but can also be a mean for tethering of organelles. In mammals and yeast, LDs have been shown to engage in MCS with nearly all organelles in the cell, while in plants only LD-ER and LD-peroxisome contacts have been characterised. We here analyse three proteins, LD-localised SEED LIPID DROPLET PROTEIN (SLDP) 1 and 2 and PM-localised LIPID DROPLET PLASMA MEMBRANE ADAPTOR. Knockout of SLDP1 and 2, as well as knockout of LIPA lead to aberrant clustering of LDs in seedlings, while ectopic co-expression of SLDP and LIPA in Nicotiana tabacum pollen tubes is sufficient to reconstitute LD-PM tethering in this tissue, where LDs normally dynamically float in the cytosol stream. We propose a model, in which SLDP and LIPA interact and thereby form a tether to anchor a subset of LDs to the PM during post-germinative growth in Arabidopsis thaliana.
HostingRepository	PRIDE
AnnounceDate	2022-06-09
AnnouncementXML	Submission_2022-06-09_03:26:30.176.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Till Ischebeck
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-26 06:19:31	ID requested
⏵ 1	2022-06-09 03:26:30	announced

Krawczyk HE, Sun S, Doner NM, Yan Q, Lim MSS, Scholz P, Niemeyer PW, Schmitt K, Valerius O, Pleskot R, Hillmer S, Braus GH, Wiermer M, Mullen RT, Ischebeck T, SEED LIPID DROPLET PROTEIN1, SEED LIPID DROPLET PROTEIN2, and LIPID DROPLET PLASMA MEMBRANE ADAPTOR mediate lipid droplet-plasma membrane tethering. Plant Cell, 34(6):2424-2448(2022) [pubmed]

submitter keyword: Arabidopsis, lipid droplets, seed, germination, seedling, TAG, membrane contact sites, tethering complex, plasma membrane

Till Ischebeck
contact affiliation	University of Göttingen, Albrecht-von-Haller-Institute for Plant Sciences and Göttingen Center for Molecular Biosciences (GZMB), Department of Plant Biochemistry, Göttingen, Germany
contact email	tischeb@gwdg.de
lab head
Till Ischebeck
contact affiliation	University of Goettingen
contact email	tischeb@gwdg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022769
     1. Label: PRIDE project
     2. Name: Identification of a lipid droplet-plasma membrane tethering complex