PXD022760

PXD022760 is an original dataset announced via ProteomeXchange.

Title	Blood exposure induces global changes in gene expression and protein secretion of B. pertussis
Description	This project was focused on transcriptome and secretome of pathogenic bacteria B. pertussis, which were cultivated on solid plates (charcoal agar) with/without blood and subsequently sub-cultured in liquid media. As a standard, Bordetella spp. strain are grown on agar plates supplemented with blood. The main aim of this project was to determine how the cells respond to blood exposure and how the blood treatment impacts on the gene expression profiles in cell inoculated from plates and subcultured in a blood-less medium. Gene expression profiles were analyzed by RNA-seq and proteins secreted by B. pertussis cells grown in liquid media were determined by LC-MS/MS technique. Our results indicate that B. pertussis cells exposed to blood secreted significantly higher amounts of several virulence factors including type III secretion system (T3SS), bifunctional hemolysin/adenylate cyclase CyaA and adhesin FhaS, compared to cells inoculated from plates without blood. Differences in secretion of T3SS needle complex, effector protein BopC and anti-sigma factor BtrA were at least an order of magnitude higher compared to the differences on the transcriptional level, suggesting that post-transcriptional regulation and/or regulation of the protein secretion plays an important role in modulating the function of T3SS in B. pertussis.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_14:14:51.284.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Denisa Petrackova
SpeciesList	scientific name: Bordetella pertussis Tohama I; NCBI TaxID: 257313;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-26 03:16:30	ID requested
⏵ 1	2021-09-09 14:14:52	announced

Drzmisek J, Stipl D, Petrackova D, Vecerek B, Dienstbier A, Identifies a Novel Essential T3SS Substrate. Int J Mol Sci, 22(2):(2021) [pubmed]

submitter keyword: Bordetella pertussis, blood, virulence factors, LFQ MS

Branislav Vecerek, PhD
contact affiliation	Laboratory of Post-Transcriptional Control of Gene Expression, Institute of Microbiology of the Czech Academy of Sciences, v.v.i.
contact email	vecerek@biomed.cas.cz
lab head
Denisa Petrackova
contact affiliation	Institute of Microbiology of the CAS, v. v. i.
contact email	petrack@biomed.cas.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD022760

PRIDE project URI

• PRIDE
  1. PXD022760
     1. Label: PRIDE project
     2. Name: Blood exposure induces global changes in gene expression and protein secretion of B. pertussis