PXD022757

PXD022757 is an original dataset announced via ProteomeXchange.

Title	Budding yeast relies on qualitative G1 cyclin specificity to couple cell cycle progression with morphogenetic development
Description	Two models have been put forward for cyclin-dependent kinase (Cdk) control of the cell cycle. In the qualitative model, cell cycle events are ordered by distinct substrate specificities associated with successive waves of G1, S and mitotic cyclins. Alternatively, the gradual quantitative rise of Cdk activity from G1 phase to mitosis could lead to ordered substrate phosphorylation at sequential thresholds. Here, we study the relative contributions of qualitative and quantitative Cdk control in the budding yeast S. cerevisiae. S-phase cyclins can be replaced by a single mitotic cyclin, albeit at the cost of reduced fitness. The single cyclin can in addition replace G1 cyclins to support ordered cell cycle progression, fulfilling key predictions of the quantitative model. However, single-cyclin cells fail to polarize or grow buds and thus cannot sustain proliferation. Our results suggest that budding yeast has become dependent on G1 cyclin specificity to couple cell cycle progression to essential morphogenetic events.
HostingRepository	PRIDE
AnnounceDate	2021-08-23
AnnouncementXML	Submission_2021-08-23_06:47:00.855.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Helen Flynn
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-26 00:27:58	ID requested
1	2021-08-23 06:38:18	announced
⏵ 2	2021-08-23 06:47:01	announced	2021-08-23: Updated publication reference for PubMed record(s): 34088668.

Pirincci Ercan D, Chr, é, tien F, Chakravarty P, Flynn HR, Snijders AP, Uhlmann F, cyclin specificity to couple cell cycle progression with morphogenetic development. Sci Adv, 7(23):(2021) [pubmed]

submitter keyword: LC-MS/MS, TMT, cell cycle, cyclin-dependent kinase, Cdk, phosphoproteomics, cyclin, yeast

Frank Uhlmann
contact affiliation	Chromosome Segregation Laboratory The Francis Crick Institute 1 Midland Road NW1 1AT UK
contact email	frank.uhlmann@crick.ac.uk
lab head
Helen Flynn
contact affiliation	The Francis Crick Institute
contact email	helen.flynn@crick.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022757
     1. Label: PRIDE project
     2. Name: Budding yeast relies on qualitative G1 cyclin specificity to couple cell cycle progression with morphogenetic development