PXD022750

PXD022750 is an original dataset announced via ProteomeXchange.

Title	A small RNA and its involvement in zinc transport
Description	The function and mode of action of small regulatory RNAs is currently still understudied in archaea. In the halophilic archaeon H. volcanii a plethora of sRNAs have been identified, however, in-depth functional analysis is missing for most of them. We selected a small RNA (s479) from H. volcanii for detailed characterization. The sRNA gene is encoded between a CRISPR RNA locus and the Cas protein gene cluster, the s479 deletion strain is viable and was characterized in detail. Transcriptome studies of wild type Haloferax cells and the deletion mutant revealed up-regulation of six genes in the deletion strain, showing that the sRNA has a distinct cellular function. Proteome comparison of wild type and deletion strains further expanded the regulon of s479 deeply rooting this sRNA within the metabolism of H. volcanii especially the regulation of transporter abundance.
HostingRepository	PRIDE
AnnounceDate	2021-08-10
AnnouncementXML	Submission_2021-08-10_06:43:45.351.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sandra Maass
SpeciesList	scientific name: Haloferax volcanii (Halobacterium volcanii); NCBI TaxID: 2246;
ModificationList	monohydroxylated residue; acetylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-25 23:55:48	ID requested
1	2021-08-10 03:50:09	announced
⏵ 2	2021-08-10 06:43:46	announced	2021-08-10: Updated project metadata.

M, ä, rkle P, Maier LK, Maa, ß S, Hirschfeld C, Bartel J, Becher D, Vo, ß B, Marchfelder A, A Small RNA Is Linking CRISPR-Cas and Zinc Transport. Front Mol Biosci, 8():640440(2021) [pubmed]

10.3389/FMOLB.2021.640440;

submitter keyword: proteomics, ABC transporter, archaea, metallostasis, transcriptomics,small RNAs

Dörte Becher
contact affiliation	Department of Microbial Proteomics, Institute of Microbiology, Center for Functional Genomics of Microbes, University of Greifswald, Felix-Hausdorff-Str. 8, 17489 Greifswald, Germany
contact email	dbecher@uni-greifswald.de
lab head
Sandra Maass
contact affiliation	University of Greifswald, Department for Microbial Proteomics
contact email	sandra.maass@uni-greifswald.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/08/PXD022750

PRIDE project URI

• PRIDE
  1. PXD022750
     1. Label: PRIDE project
     2. Name: A small RNA and its involvement in zinc transport