PXD022749

PXD022749 is an original dataset announced via ProteomeXchange.

Title	Differential proteomic analysis reveals the mechanism of Providencia.sp to selenium and manganese stress
Description	In this study, iTRAQ technique is employed to reveal the proteomic response of Providencia. sp under selenium and manganese stress. After trypsin digestion, peptide was desalted by Strata X C18 SPE column (Phenomenex) and vacuum-dried. Peptide was reconstituted in 0.5 M TEAB and processed according to the manufacturer’s protocol for TMT kit/iTRAQ kit. Then, The tryptic peptides were fractionated into fractions by high pH reverse-phase HPLC using Agilent 300Extend C18 column. Finally, The peptides were subjected to NSI source followed by tandem mass spectrometry (MS/MS) in Q ExactiveTM Plus (Thermo) coupled online to the UPLC.
HostingRepository	iProX
AnnounceDate	2020-11-26
AnnouncementXML	Submission_2020-11-25_22:16:10.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	ShengWei Huang
SpeciesList	scientific name: Providencia rettgeri 947; NCBI TaxID: 1255575;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-25 22:14:39	ID requested
⏵ 1	2020-11-25 22:16:10	announced

Dataset with its publication pending

submitter keyword: Providencia. sp, selenium, manganese, iTRAQ

LiFang wu
contact affiliation	Hefei Institutes of Physical Science, Chinese Academy of Sciences
contact email	lfwu@ipp.ac.cn
lab head
ShengWei Huang
contact affiliation	Hefei Institutes of Physical Science, Chinese Academy of Sciences
contact email	swhuang@ipp.ac.cn
dataset submitter

iProX dataset URI