PXD022739

PXD022739 is an original dataset announced via ProteomeXchange.

Title	The M-phase regulatory phosphatase PP2A-B55δ opposes protein kinase A on Arpp19 to initiate meiotic division
Description	Oocytes are held in meiotic prophase for prolonged periods until hormonal signals trigger meiotic divisions. Key players of M-phase entry are the opposing Cdk1 kinase and PP2AB55δ phosphatase. In Xenopus, the protein Arpp19, phosphorylated at serine 67 by Greatwall, plays an essential role in inhibiting PP2A-B55δ, promoting Cdk1 activation. Furthermore Arpp19 has an earlier role in maintaining the prophase arrest through a second serine (S109) phosphorylated by PKA. Prophase release, induced by progesterone, relies on Arpp19 dephosphorylation at S109, owing to an unknown phosphatase. Here we identified this phosphatase as PP2A-B55δ. In prophase, PKA and PP2A-B55δ are simultaneously active, suggesting the presence of other important targets for both enzymes. The drop in PKA activity induced by progesterone decreases S109 phosphorylation, unlocking the prophase block. Hence, PP2A-B55δ acts critically on Arpp19 on two distinct sites, opposing PKA and Greatwall to orchestrate the prophase release and M-phase entry.
HostingRepository	PRIDE
AnnounceDate	2021-03-12
AnnouncementXML	Submission_2021-04-06_03:05:46.217.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Laurent Lignières
SpeciesList	scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355;
ModificationList	phosphorylated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-25 06:41:06	ID requested
1	2021-03-12 04:56:16	announced
⏵ 2	2021-04-06 03:05:47	announced	2021-04-06: Updated publication reference for PubMed record(s): 33758202.

Lemonnier T, Daldello EM, Poulhe R, Le T, Miot M, Ligni, è, res L, Jessus C, Dupr, é A, opposes protein kinase A on Arpp19 to initiate meiotic division. Nat Commun, 12(1):1837(2021) [pubmed]

submitter keyword: Arpp19, M-phase, meiotic, PP2A, B55, Xenopus, Oocyte, phosphorylation, phosphatase

Guillaume Chevreux
contact affiliation	Proteomic facility of Institut Jacques Monod, UMR7592 (CNRS and Paris University), 75013 Paris, France (lab head)
contact email	guillaume.chevreux@ijm.fr
lab head
Laurent Lignières
contact affiliation	Proteomic facility of Institut Jacques Monod - CNRS & Université de Paris
contact email	laurent.lignieres@ijm.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD022739

PRIDE project URI

• PRIDE
  1. PXD022739
     1. Label: PRIDE project
     2. Name: The M-phase regulatory phosphatase PP2A-B55δ opposes protein kinase A on Arpp19 to initiate meiotic division