PXD022735

PXD022735 is an original dataset announced via ProteomeXchange.

Title	In-depth proteomic analysis of Plasmodium berghei sporozoites using trapped ion mobility spectrometry with parallel accumulation-serial fragmentation
Description	We took advantage of a novel highly sensitive timsTOF PRO mass spectrometer, based on trapped ion mobility spectrometry with parallel accumulation-serial fragmentation. Combined with three alternative methods for sporozoite purification, this approach allowed us to identify the deep proteome of P. berghei sporozoites using low numbers of parasites. This study provides a reference proteome for P. berghei sporozoites, identifying a core set of proteins expressed accross species, and illustrates how the unprecedented sensitivity of the timsTOF PRO system enables deep proteomic analysis from limited sample amounts.
HostingRepository	PRIDE
AnnounceDate	2021-02-11
AnnouncementXML	Submission_2021-02-11_14:28:40.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cédric Pionneau
SpeciesList	scientific name: Plasmodium berghei ANKA; NCBI TaxID: 5823;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-25 06:29:57	ID requested
⏵ 1	2021-02-11 14:28:41	announced

Hamada S, Pionneau C, Parizot C, Silvie O, Chardonnet S, Marinach C, In-depth proteomic analysis of Plasmodium berghei sporozoites using trapped ion mobility spectrometry with parallel accumulation-serial fragmentation. Proteomics, 21(6):e2000305(2021) [pubmed]

submitter keyword: Malaria, sporozoites, Plasmodium berghei, proteome, mass spectrometry, timsTOF PRO

Solenne Chardonnet
contact affiliation	Plateforme P3S - UMS PASS 37 - Sorbonne Universite - INSERM
contact email	solenne.chardonnet@sorbonne-universite.fr
lab head
Cédric Pionneau
contact affiliation	Plateforme P3S UMS-37 PASS – Sorbonne Universite
contact email	cedric.pionneau@sorbonne-universite.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD022735

PRIDE project URI

• PRIDE
  1. PXD022735
     1. Label: PRIDE project
     2. Name: In-depth proteomic analysis of Plasmodium berghei sporozoites using trapped ion mobility spectrometry with parallel accumulation-serial fragmentation