PXD022731

PXD022731 is an original dataset announced via ProteomeXchange.

Title	Dysfunction of CEP128 impairs spermatogenesis but not ciliogenesis in humans and mice
Description	The centrosome is a conserved eukaryotic organelle essential for reproductive process, and centrosomal proteins (CEP) are necessary for composition of the centrosome. However, few CEPs have been genetically linked to fertility, and the related molecular mechanisms remains mysterious. Here, we identified a new CEP, CEP128, which is functional in spermatogenesis, fertilization and embryonic development in both humans and mice. The variants of CEP128/Cep128 could lead to aberrant centrosome structures of the sperm inducing to anomalies in sperm morphology, count as well as motility, and further result in male infertility, but did not grossly affect ciliogenesis. Mechanistically, both loss and up-regulation of CEP128 could cause suppressed expressions of the genes involved in the spermatogenesis and fertilization phase. Altogether, our findings unprecedentedly unveil a crucial role of CEP128 in male fertility and provides new insight into the function of CEPs in human disease.
HostingRepository	PRIDE
AnnounceDate	2022-05-19
AnnouncementXML	Submission_2022-05-19_12:41:19.492.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jichang Huang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-25 04:24:13	ID requested
⏵ 1	2022-05-19 12:41:20	announced

Zhang X, Wang L, Ma Y, Wang Y, Liu H, Liu M, Qin L, Li J, Jiang C, Zhang X, Shan X, Liu Y, Li J, Li Y, Zheng R, Sun Y, Sun J, Leng X, Liang Y, Zhang F, Jiang X, Yang Y, Shen Y, CEP128 is involved in spermatogenesis in humans and mice. Nat Commun, 13(1):1395(2022) [pubmed]

submitter keyword: CEP128

infertility

spermatogenesis

centrosome

Ying Shen
contact affiliation	West China Second University Hospital, Sichuan University
contact email	yingcaishen01@163.com
lab head
Jichang Huang
contact affiliation	School of Life Sciences, Fudan University
contact email	14110700074@fudan.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD022731

PRIDE project URI

• PRIDE
  1. PXD022731
     1. Label: PRIDE project
     2. Name: Dysfunction of CEP128 impairs spermatogenesis but not ciliogenesis in humans and mice