PXD022717

PXD022717 is an original dataset announced via ProteomeXchange.

Title	SMC Complexes Are Guarded by the SUMO Protease Ulp2 Against SUMO-Chain-Mediated Turnover
Description	Structural maintenance of chromosomes (SMC) complexes, cohesin, condensin and Smc5/6, are essential for viability and participate in multiple processes, including sister chromatid cohesion, chromosome condensation, and DNA repair. Here we show that SUMO chains target all three SMC complexes and are antagonized by the SUMO protease Ulp2 to prevent their turnover. We uncover that the essential role of the cohesin-associated subunit Pds5 is to counteract SUMO chains jointly with Ulp2. Importantly, fusion of Ulp2 to kleisin Scc1 supports viability of PDS5 null cells and protects cohesin from proteasomal degradation mediated by the SUMO-targeted ubiquitin ligase Slx5/Slx8. The lethality of PDS5 deleted cells can also be bypassed by simultaneous loss of the PCNA unloader, Elg1, and the cohesin releaser, Wpl1, but only when Ulp2 is functional. Condensin and Smc5/6 complex are similarly guarded by Ulp2 against unscheduled SUMO-chain assembly, which we propose to time the availability of SMC complexes on chromatin.
HostingRepository	PRIDE
AnnounceDate	2021-08-05
AnnouncementXML	Submission_2021-08-05_09:09:39.171.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ivan Psakhye
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	sumoylated lysine; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-25 03:03:19	ID requested
⏵ 1	2021-08-05 09:09:39	announced

Psakhye I, Branzei D, SMC complexes are guarded by the SUMO protease Ulp2 against SUMO-chain-mediated turnover. Cell Rep, 36(5):109485(2021) [pubmed]

submitter keyword: Cohesin, Condensin, Smc5/6, SMC complexes, Pds5, SUMO, SENP/Ulp proteases, SUMO chains, STUbL-mediated proteasomal degradation, Slx5/8

Dana Branzei
contact affiliation	IFOM Foundation - The FIRC Institute of Molecular Oncology Foundation Via Adamello, 16 20139 Milan, Italy Consiglio Nazionale delle Ricerche-IGM Via Abbiategrasso,207, 27100 Pavia
contact email	dana.branzei@ifom.eu
lab head
Ivan Psakhye
contact affiliation	IFOM
contact email	ivan.psakhye@ifom.eu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022717
     1. Label: PRIDE project
     2. Name: SMC Complexes Are Guarded by the SUMO Protease Ulp2 Against SUMO-Chain-Mediated Turnover