PXD022712

PXD022712 is an original dataset announced via ProteomeXchange.

Title	Integrative analysis of two russeted and non-russeted closely related apple varieties during fruit development provides new insights about the cuticle/suberin interplay
Description	Apple russeting is characterized by the deposition of suberin in the inner part of the epidermal cell walls. It develops at the fruit surface when the integrity of the cuticle has been lost. Cutin and wax composition (including triterpene profile) is also affected in russeted skin. In the present work, the fruit growth kinetic of two closely related apple varieties, ‘Canada Gris’ and ‘Canada Blanc’, which display russeted and waxy skin phenotypes, respectively, was investigated. We used a combination of transcriptomic, proteomic, and metabolomic approaches to better understand the molecular events associated with cuticle failure and suberization. A particular interest was also given to the triterpene specifically found in russeted skins and their synthesis. Our results indicate that the decreased synthesis of cutin and wax, observed in russeted skin throughout fruit development, is a determining factor for russeting. We identified a lipid transfer protein (LTP-3) as a potential key player in cuticle formation. Metabolomics highlighted a large diversity of triterpene-hydroxycinnamates in russeted tissues whose accumulation was highly correlated with suberin-related genes, including some enzymes belonging to the BAHD (HXXXD-motif) acyltransferase family. This study shed light for the first time on the crosstalk between triterpene and suberin pathways.
HostingRepository	PRIDE
AnnounceDate	2022-02-22
AnnouncementXML	Submission_2022-02-22_04:20:27.969.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Céline LECLERCQ
SpeciesList	scientific name: Malus domestica; NCBI TaxID: 3750;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetic acid derivatized residue
Instrument	TripleTOF 5600+

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-24 09:04:36	ID requested
⏵ 1	2022-02-22 04:20:28	announced

Andr, é CM, Guerriero G, Lateur M, Charton S, Leclercq CC, Renaut J, Hausman JF, Legay S, Identification of Novel Candidate Genes Involved in Apple Cuticle Integrity and Russeting-Associated Triterpene Synthesis Using Metabolomic, Proteomic, and Transcriptomic Data. Plants (Basel), 11(3):(2022) [pubmed]

submitter keyword: : russeting, integrative analysis, LC-MS/MS, metabolomics, conjugated triterpene, suberin, apple skin.

Céline LECLERCQ
contact affiliation	LIST-ERIN-ERTP-Green Tech platform Belvaux, LUXEMBOURG
contact email	celine.leclercq@list.lu
lab head
Céline LECLERCQ
contact affiliation	LIST-ERIN-ERTP-GreenTech platform
contact email	celine.leclercq@list.lu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022712
     1. Label: PRIDE project
     2. Name: Integrative analysis of two russeted and non-russeted closely related apple varieties during fruit development provides new insights about the cuticle/suberin interplay