PXD022709

PXD022709 is an original dataset announced via ProteomeXchange.

Title	Proteomics and phosphoproteomics of SARS-CoV-2 and IAV treated APCs
Description	The clinical course of SARS-CoV-2 infection is highly variable with a subset of patients developing severe COVID-19 and acute respiratory distress syndrome (ARDS). COVID-19 induced lung injury and respiratory failure appears to be driven by dysregulated immune responses, yet the exact mechanisms remain unknown. Here, we analyzed monocytes isolated from healthy donors treated with SARS-CoV-2, influenza A (Panama strain) or TLR7/8 agonist R848. Notably, overnight exposure to SARS-CoV-2, but not influenza A virus, induced a profibrotic signature, characterized by high expression of known fibrogenic factors like TGFB1, SPP1 and LGMN, and showed highly significant similarity with profibrotic macrophage populations identified in idiopathic pulmonary fibrosis (IPF). In conclusion, SARS-CoV-2 triggers profibrotic macrophage responses, and ARDS-associated lung fibrosis.
HostingRepository	PRIDE
AnnounceDate	2021-11-29
AnnouncementXML	Submission_2021-11-29_06:37:42.217.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tommaso Mari
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Influenza A virus (A/Panama/2007/1999(H3N2)); NCBI TaxID: 381513; scientific name: Severe acute respiratory syndrome coronavirus 2; NCBI TaxID: 2697049;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-24 07:56:37	ID requested
⏵ 1	2021-11-29 06:37:42	announced

Dataset with its publication pending

submitter keyword: PBMC, APC, SARS-CoV-2, IAV, TMT, phosphoproteomics

Matthias Selbach
contact affiliation	Proteome Dynamics, Max Delbruck Center for Molecular Medicine, Germany
contact email	matthias.selbach@mdc-berlin.de
lab head
Tommaso Mari
contact affiliation	Max Delbrück Center for Molecular Medicine - MDC
contact email	tommaso.mari@mdc-berlin.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022709
     1. Label: PRIDE project
     2. Name: Proteomics and phosphoproteomics of SARS-CoV-2 and IAV treated APCs