PXD022700

PXD022700 is an original dataset announced via ProteomeXchange.

Title	Toxoplasma gondii; protein posttranslational modification; lactylation; metabolism; function
Description	Toxoplasma gondii, a single-celled protozoan parasite (phylum Apicomplexa) and remains the causative pathogen of one of the most wide-spread infectious diseases, toxoplasmosis. To continuously proliferate in warm-blooded animals, this parasite undergoes repeated cycles of invasion, division and induction of host cell rupture. Lactate, which is derived from cellular metabolic pathways, is widely considered not only an energy source in organisms but also a regulatory molecule that participates in gene activation. Lysine lactylation is a new type of protein posttranslational modification (PTM) that was recently associated with chromatin remodeling, but the lysine lactylation of histone and nonhistone proteins has not yet been studied in parasites. To examine the prevalence and function of lactylation in T. gondii parasites, we mapped the lactylation proteome of proliferating tachyzoite cells and found 1,964 lactylation sites on 955 proteins in the T. gondii RH strain. The lactylated proteins were distributed in multiple subcellular compartments and were closely related to a wide variety of biological processes, including mRNA splicing, the citrate cycle (TCA cycle), aminoacyl-tRNA biosynthesis, glyoxylate and dicarboxylate metabolism, glycolysis/gluconeogenesis, oxidative phosphorylation, RNA transport and the HIF-1 signaling pathway. Lysine lactation might regulate numerous cellular processes to aid the establishment of a hospitable environment that allows the survival, replication and dissemination of the parasite. These study offers the first data of the global lactylation proteome and provides a basis for further functional dissection of important proteins associated with T. gondii development and pathogenicity.
HostingRepository	PRIDE
AnnounceDate	2024-04-07
AnnouncementXML	Submission_2024-04-06_17:08:04.204.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qijun Chen
SpeciesList	scientific name: Toxoplasma gondii; NCBI TaxID: 5811;
ModificationList	lactic acid
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-24 07:52:03	ID requested
⏵ 1	2024-04-06 17:08:05	announced

10.1016/j.gpb.2022.09.010;

Yin D, Jiang N, Cheng C, Sang X, Feng Y, Chen R, Chen Q, Protein Lactylation and Metabolic Regulation of the Zoonotic Parasite Toxoplasma gondii. Genomics Proteomics Bioinformatics, 21(6):1163-1181(2023) [pubmed]

submitter keyword: Toxoplasma gondii

Qijun Chen
contact affiliation	Key Laboratory of Animal Infectious Diseases in Northeast China, Ministry of Education; Key Laboratory of Zoonosis, Shenyang Agricultural University, 120 Dongling Road, Shenyang 110166, China.
contact email	qijunchen759@syau.edu.cn
lab head
Qijun Chen
contact affiliation	Shenyang Agricultural University
contact email	qijunchen759@syau.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/04/PXD022700

PRIDE project URI

• PRIDE
  1. PXD022700
     1. Label: PRIDE project
     2. Name: Toxoplasma gondii; protein posttranslational modification; lactylation; metabolism; function