PXD022695 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The self-peptide repertoire plays a critical role in transplant tolerance induction |
Description | While direct allorecognition underpins both solid organ allograft rejection and tolerance induction, the specific molecular targets of most directly-alloreactive CD8+ T cells have not been defined. In this study, we used a combination of genetically-engineered MHC I constructs, mice with a hepatocyte-specific mutation in the class I antigen-presentation pathway and immunopeptidomic analysis to provide definitive evidence for the contribution of the peptide cargo of allogeneic MHC I molecules to transplant tolerance induction. We established a systematic approach for the discovery of directly-recognised pMHC epitopes, and identified 17 strongly immunogenic H-2Kb-associated peptides recognised by CD8+ T cells from B10.BR (H-2k) mice, 13 of which were also recognised by BALB/c (H-2d) mice. As few as five different tetramers used together were able to identify almost 40% of alloreactive T cells within a polyclonal population. To our knowledge, this represents the first example of such an approach in the context of direct allorecognition. |
HostingRepository | PRIDE |
AnnounceDate | 2021-12-06 |
AnnouncementXML | Submission_2021-12-05_23:46:51.831.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD022695 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Pouya Faridi |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | monohydroxylated residue; deamidated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-11-24 07:51:02 | ID requested | |
⏵ 1 | 2021-12-05 23:46:52 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Immunopeptidomics, HLA, Transplantation |
Contact List
Anthony Purcell |
contact affiliation | Deputy Head (Research), Department of Biochemistry and Molecular Biology Head Immunoproteomics Laboratory Infection and Immunity Program Room 214, level 2, 15 Innovation Walk Monash Biomedicine Discovery Institute Monash University, Clayton Campus Clayton 3800 Victoria Australia |
contact email | anthony.purcell@monash.edu |
lab head | |
Pouya Faridi |
contact affiliation | Monash University |
contact email | pouya.faridi@monash.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD022695
- Label: PRIDE project
- Name: The self-peptide repertoire plays a critical role in transplant tolerance induction