PXD022694 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A genetic platform to delineate tissue-specific in vivo secretion |
Description | At present, it remains difficult to deconvolute serum in order to identify the cell or tissue origin of a given circulating protein. Here, by exploiting the properties of proximity biotinylation, we describe a mouse model that enables the elucidation of the in vivo tissue-specific secretome. As an example, we demonstrate how we can readily identify in vivo endothelial-specific secretion, as well as how this model allows for the characterization of muscle-derived serum proteins that either increase or decrease with exercise. This genetic platform should therefore be of wide utility in understanding normal and disease physiology and for the rational design of tissue-specific disease biomarkers. |
HostingRepository | PRIDE |
AnnounceDate | 2021-01-14 |
AnnouncementXML | Submission_2021-01-14_00:35:46.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD022694 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Mehdi Pirooznia |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-11-24 07:50:55 | ID requested | |
⏵ 1 | 2021-01-14 00:35:47 | announced | |
Publication List
Liu J, Jang JY, Pirooznia M, Liu S, Finkel T, The secretome mouse provides a genetic platform to delineate tissue-specific in vivo secretion. Proc Natl Acad Sci U S A, 118(3):(2021) [pubmed] |
Keyword List
submitter keyword: Secretome, biotinylation, ER-BioIDHA, serum, endothelial cells, muscle |
Contact List
Toren Finkel |
contact affiliation | Aging Institute, University of Pittsburgh School of Medicine/UPMC |
contact email | finkelt@pitt.edu |
lab head | |
Mehdi Pirooznia |
contact affiliation | National Heart Lung and Blood Institute , National Institutes of Health |
contact email | mehdi.pirooznia@nih.gov |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD022694 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD022694
- Label: PRIDE project
- Name: A genetic platform to delineate tissue-specific in vivo secretion