PXD022694

PXD022694 is an original dataset announced via ProteomeXchange.

Title	A genetic platform to delineate tissue-specific in vivo secretion
Description	At present, it remains difficult to deconvolute serum in order to identify the cell or tissue origin of a given circulating protein. Here, by exploiting the properties of proximity biotinylation, we describe a mouse model that enables the elucidation of the in vivo tissue-specific secretome. As an example, we demonstrate how we can readily identify in vivo endothelial-specific secretion, as well as how this model allows for the characterization of muscle-derived serum proteins that either increase or decrease with exercise. This genetic platform should therefore be of wide utility in understanding normal and disease physiology and for the rational design of tissue-specific disease biomarkers.
HostingRepository	PRIDE
AnnounceDate	2021-01-14
AnnouncementXML	Submission_2021-01-14_00:35:46.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD022694
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Mehdi Pirooznia
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-24 07:50:55	ID requested
⏵ 1	2021-01-14 00:35:47	announced

Liu J, Jang JY, Pirooznia M, Liu S, Finkel T, The secretome mouse provides a genetic platform to delineate tissue-specific in vivo secretion. Proc Natl Acad Sci U S A, 118(3):(2021) [pubmed]

submitter keyword: Secretome, biotinylation, ER-BioIDHA, serum, endothelial cells, muscle

Toren Finkel
contact affiliation	Aging Institute, University of Pittsburgh School of Medicine/UPMC
contact email	finkelt@pitt.edu
lab head
Mehdi Pirooznia
contact affiliation	National Heart Lung and Blood Institute , National Institutes of Health
contact email	mehdi.pirooznia@nih.gov
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD022694

PRIDE project URI

• PRIDE
  1. PXD022694
     1. Label: PRIDE project
     2. Name: A genetic platform to delineate tissue-specific in vivo secretion