PXD022685 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Comparative Proteomics Analysis Reveals Unique Early Signaling Response of Saccharomyces cerevisiae to Oxidants with Different Mechanism of Action Prajita Pandey 1,‡, Khadiza Zaman 2, ‡, Laszlo Prokai 2 , and Vladimir Shulaev 1,* |
Description | The early signaling events involved in oxidant recognition and triggering of oxidant-specific defense mechanisms to counteract oxidative stress still remains largely elusive. Our discovery driven comparative proteomics analysis revealed unique early signaling response of yeast Saccharomyces cerevisiae on proteome level to oxidants with different mechanism of action as early as 3 min after treatment with four oxidants, namely H2O2, cumene hydroperoxide (CHP), menadione and diamide, when protein abundances were compared using label-free quantification relying on a high-resolution mass analyzer (Orbitrap). We identified significant regulation of 196 proteins in response to H2O2, 569 proteins in response to CHP, 369 proteins in response to menadione and 207 proteins in response to diamide. Only 17 proteins were common across all treatments, but several more proteins were shared between two or three oxidants. Pathway analyses revealed that each oxidant triggered unique signaling mechanism associated with cell survival and repair. Signaling pathways mostly regulated by oxidants were Ran, mTOR, Rho, and eIEF2. Furthermore, each oxidant regulated these pathways in a unique way indicating specificity of response to oxidants having different mode of action. We hypothesize that interplay of these signaling pathway may be important in recognizing different oxidants to trigger different downstream MAPK signaling cascades and induce specific responses. |
HostingRepository | PRIDE |
AnnounceDate | 2021-01-04 |
AnnouncementXML | Submission_2021-01-04_05:21:13.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Laszlo Prokai |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-11-24 01:24:34 | ID requested | |
⏵ 1 | 2021-01-04 05:21:14 | announced | |
Publication List
Pandey P, Zaman K, Prokai L, Shulaev V, to Oxidants with Different Mechanism of Action. Int J Mol Sci, 22(1):(2020) [pubmed] |
Keyword List
submitter keyword: oxidative stress |
reactive oxygen species |
antioxidants |
global untargeted proteomics |
TOR signaling |
RAN signaling |
MAPK cascade |
Contact List
Dr. Vladimir Shulaev |
contact affiliation | 1 Department of Biological Sciences, College of Arts and Sciences, University of North Texas, Denton, TX 2 Advanced Environmental Research Institute (AERI), University of North Texas, Denton, TX |
contact email | Vladimir.Shulaev@unt.edu |
lab head | |
Laszlo Prokai |
contact affiliation | Department of Pharmacology & Neuroscience UNT Health Science Center Fort Worth, TX, USA |
contact email | advancedms@unthsc.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD022685
- Label: PRIDE project
- Name: Comparative Proteomics Analysis Reveals Unique Early Signaling Response of Saccharomyces cerevisiae to Oxidants with Different Mechanism of Action Prajita Pandey 1,‡, Khadiza Zaman 2, ‡, Laszlo Prokai 2 , and Vladimir Shulaev 1,*