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PXD022685

PXD022685 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleComparative Proteomics Analysis Reveals Unique Early Signaling Response of Saccharomyces cerevisiae to Oxidants with Different Mechanism of Action Prajita Pandey 1,‡, Khadiza Zaman 2, ‡, Laszlo Prokai 2 , and Vladimir Shulaev 1,*
DescriptionThe early signaling events involved in oxidant recognition and triggering of oxidant-specific defense mechanisms to counteract oxidative stress still remains largely elusive. Our discovery driven comparative proteomics analysis revealed unique early signaling response of yeast Saccharomyces cerevisiae on proteome level to oxidants with different mechanism of action as early as 3 min after treatment with four oxidants, namely H2O2, cumene hydroperoxide (CHP), menadione and diamide, when protein abundances were compared using label-free quantification relying on a high-resolution mass analyzer (Orbitrap). We identified significant regulation of 196 proteins in response to H2O2, 569 proteins in response to CHP, 369 proteins in response to menadione and 207 proteins in response to diamide. Only 17 proteins were common across all treatments, but several more proteins were shared between two or three oxidants. Pathway analyses revealed that each oxidant triggered unique signaling mechanism associated with cell survival and repair. Signaling pathways mostly regulated by oxidants were Ran, mTOR, Rho, and eIEF2. Furthermore, each oxidant regulated these pathways in a unique way indicating specificity of response to oxidants having different mode of action. We hypothesize that interplay of these signaling pathway may be important in recognizing different oxidants to trigger different downstream MAPK signaling cascades and induce specific responses.
HostingRepositoryPRIDE
AnnounceDate2021-01-04
AnnouncementXMLSubmission_2021-01-04_05:21:13.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLaszlo Prokai
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-11-24 01:24:34ID requested
12021-01-04 05:21:14announced
Publication List
Pandey P, Zaman K, Prokai L, Shulaev V, to Oxidants with Different Mechanism of Action. Int J Mol Sci, 22(1):(2020) [pubmed]
Keyword List
submitter keyword: oxidative stress
reactive oxygen species
antioxidants
global untargeted proteomics
TOR signaling
RAN signaling
MAPK cascade
Contact List
Dr. Vladimir Shulaev
contact affiliation1 Department of Biological Sciences, College of Arts and Sciences, University of North Texas, Denton, TX 2 Advanced Environmental Research Institute (AERI), University of North Texas, Denton, TX
contact emailVladimir.Shulaev@unt.edu
lab head
Laszlo Prokai
contact affiliationDepartment of Pharmacology & Neuroscience UNT Health Science Center Fort Worth, TX, USA
contact emailadvancedms@unthsc.edu
dataset submitter
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Dataset FTP location
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